Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 63% activation at 10 mM | Cinchona succirubra | |
EDTA | 30% activation at 1 mM | Cinchona succirubra |
General Stability | Organism |
---|---|
in absence of SH-group protectors, 50% loss of activity after 48 h, in presence of 14 mM 2-mercaptoethanol, 50% loss of activity after 2 weeks | Cinchona succirubra |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 60% inhibition at 1 mM | Cinchona succirubra | |
Cu2+ | 50% inhibition at 1 mM | Cinchona succirubra | |
iodoacetamide | 60% inhibition at 1 mM | Cinchona succirubra | |
iodoacetate | 30% inhibition at 1 mM | Cinchona succirubra | |
Mn2+ | 45% inhibition at 10 mM | Cinchona succirubra | |
N-ethylmaleimide | 92% inhibition at 1 mM | Cinchona succirubra | |
p-chloromercuribenzoate | 47% inhibition at 1 mM, 10 mM dithiothreitol protects | Cinchona succirubra | |
phenylmercuric acetate | 80% inhibition at 1 mM, 10 mM dithiothreitol protects | Cinchona succirubra | |
Zn2+ | 77% inhibition at 1 mM | Cinchona succirubra |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
emodin | isoform I | Cinchona succirubra | |
0.01 | - |
anthrapurpurin | isoform II | Cinchona succirubra | |
0.01 | - |
quinizarin | isoform III | Cinchona succirubra | |
0.01 | - |
2,6-dihydroxyanthracene-9,10-dione | isoform IV | Cinchona succirubra | |
0.01 | - |
1,8-dihydroxyanthraquinone | isoform V | Cinchona succirubra |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no cation requirement | Cinchona succirubra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Cinchona succirubra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a hydroxyanthraquinone | Cinchona succirubra | a range of anthraquinones and some flavones can act as acceptors | UDP + a glucosyloxyanthraquinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cinchona succirubra | - |
5 distinct glucosylating activities with different pI values and substrate specificities, named isoforms I-V | - |
Purification (Comment) | Organism |
---|---|
- |
Cinchona succirubra |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Cinchona succirubra | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cinchona succirubra |
Storage Stability | Organism |
---|---|
-20°C, 25 mM histidine/HCl buffer, pH 7.0, 10% glycerol, 10 mM dithioerythritol, stable for 2 months | Cinchona succirubra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 1,8-dihydroxyanthraquinone | - |
Cinchona succirubra | UDP + 1-O-beta-D-glucopyranosyl-1,8-dihydroxyanthraquinone | - |
? | |
UDP-alpha-D-glucose + 2,6-dihydroxyanthracene-9,10-dione | - |
Cinchona succirubra | UDP + 6-hydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + anthrapurpurin | - |
Cinchona succirubra | UDP + 2,7-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + emodin | - |
Cinchona succirubra | UDP + 7,8-dihydroxy-3-methyl-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside | - |
? | |
UDP-alpha-D-glucose + quinizarin | - |
Cinchona succirubra | UDP + 4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside | - |
? | |
UDP-glucose + a hydroxyanthraquinone | a range of anthraquinones and some flavones can act as acceptors | Cinchona succirubra | UDP + a glucosyloxyanthraquinone | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cinchona succirubra |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for isoforms I-V | Cinchona succirubra |