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Literature summary for 2.4.1.180 extracted from
- The cell wall teichuronic acid synthetase (TUAS) is an enzyme complex located in the cytoplasmic membrane of Micrococcus luteus (2010), Biochem. Res. Int., 2010, 395758.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Micrococcus luteus | 5618 | - |
| plasma membrane | - |
Micrococcus luteus | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52500 | - |
4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE | Micrococcus luteus |
| 54000 | - |
4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE | Micrococcus luteus |
| 440000 | - |
TUAS complex | Micrococcus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native TUAS complex partially by fractionation of Thesit-solubilized membrane fraction with ammonium sulfate | Micrococcus luteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
TUAS embedded in the cell wall, pH 8.2, 25°C | Micrococcus luteus |
| 8.7 | - |
TUAS embedded in the vesicle membrane, pH 8.2, 25°C | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-mannosaminuronate + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | - |
Micrococcus luteus | UDP + N-acetyl-beta-D-mannosaminuronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | when the eight subunits disassociate, the protein loses its conformation as well as the catalytic function for TUA biosynthesis | Micrococcus luteus |
| octamer | 4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE | Micrococcus luteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ManNAcA-transferase | - |
Micrococcus luteus |
| teichuronic acid synthetase | - |
Micrococcus luteus |
| TUAS | - |
Micrococcus luteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Micrococcus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Micrococcus luteus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the 440 kDa enzyme complex, named teichuronic acid synthetase, TUAS, is an octomer composed of two kinds of glycosyltransferases, glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS is a bifunctional glycosyltransferase capable of transferring both the component sugars, glucose and ManNAcA, for the TUA polymer. The purified TUAS contains carotinoids and lipids. TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane | Micrococcus luteus |
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Release 2023.1 (January 2023)
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