Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii OT3 |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor-diffusion method, pH 9.3, crystals belong to P2(1) space group | Pyrococcus horikoshii OT3 |
Protein Variants | Comment | Organism |
---|---|---|
delN238-S247 | loop-truncated mutant exhibits a 2-fold increase in activity relative to the wild type enzyme. The branching activity of the deletion variant is decreased. In the wild type enzyme, the degree of polymerization of the reaction products has peaks ranging from 7 to 12, while the loop-truncated mutant has peaks in the range of degree of polymerization 10 to 13, indicating that the chain lengths of the reaction products are slightly longer than that of the wild type enzyme. The flexible loop is associated with the catalytic process of GH57 glycogen branching enzymes and plays an important role in the branching activity and the variable lengths of the branches | Pyrococcus horikoshii OT3 |
E185Q | enzymatic activity is abolished | Pyrococcus horikoshii OT3 |
W22A | complete loss of activity | Pyrococcus horikoshii OT3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii OT3 | O50094 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose | the glycogen branching enzyme catalyzes the formation of alpha-1,6-branching points during glycogenesis by cleaving alpha-1,4 bonds and making new alpha-1,6 bonds. In the wild type enzyme, the degree of polymerization of the reaction products has peaks ranging from 7 to 12, while the loop-truncated mutant enzyme has peaks in the range of degree of polymerization of 10 to 13, indicating that the chain lengths of the reaction products are slightly longer than that of the wild type enzyme. The flexible loop is associated with the catalytic process of GH57 glycogen branching enzymes and plays an important role in the branching activity and the variable lengths of the branches | Pyrococcus horikoshii OT3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH57-type glycogen branching enzyme | - |
Pyrococcus horikoshii OT3 |
PH1386 | - |
Pyrococcus horikoshii OT3 |
PhGBE | - |
Pyrococcus horikoshii OT3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Pyrococcus horikoshii OT3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay az | Pyrococcus horikoshii OT3 |
General Information | Comment | Organism |
---|---|---|
metabolism | the glycogen branching enzyme catalyzes the formation of alpha-1,6-branching points during glycogenesis by cleaving alpha-1,4 bonds and making new alpha-1,6 bonds | Pyrococcus horikoshii OT3 |