Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.18 extracted from

  • Na, S.; Park, M.; Jo, I.; Cha, J.; Ha, N.
    Structural basis for the transglycosylase activity of a GH57-type glycogen branching enzyme from Pyrococcus horikoshii (2017), Biochem. Biophys. Res. Commun., 484, 850-856 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii OT3

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method, pH 9.3, crystals belong to P2(1) space group Pyrococcus horikoshii OT3

Protein Variants

Protein Variants Comment Organism
delN238-S247 loop-truncated mutant exhibits a 2-fold increase in activity relative to the wild type enzyme. The branching activity of the deletion variant is decreased. In the wild type enzyme, the degree of polymerization of the reaction products has peaks ranging from 7 to 12, while the loop-truncated mutant has peaks in the range of degree of polymerization 10 to 13, indicating that the chain lengths of the reaction products are slightly longer than that of the wild type enzyme. The flexible loop is associated with the catalytic process of GH57 glycogen branching enzymes and plays an important role in the branching activity and the variable lengths of the branches Pyrococcus horikoshii OT3
E185Q enzymatic activity is abolished Pyrococcus horikoshii OT3
W22A complete loss of activity Pyrococcus horikoshii OT3

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii OT3 O50094
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose the glycogen branching enzyme catalyzes the formation of alpha-1,6-branching points during glycogenesis by cleaving alpha-1,4 bonds and making new alpha-1,6 bonds. In the wild type enzyme, the degree of polymerization of the reaction products has peaks ranging from 7 to 12, while the loop-truncated mutant enzyme has peaks in the range of degree of polymerization of 10 to 13, indicating that the chain lengths of the reaction products are slightly longer than that of the wild type enzyme. The flexible loop is associated with the catalytic process of GH57 glycogen branching enzymes and plays an important role in the branching activity and the variable lengths of the branches Pyrococcus horikoshii OT3 ?
-
?

Synonyms

Synonyms Comment Organism
GH57-type glycogen branching enzyme
-
Pyrococcus horikoshii OT3
PH1386
-
Pyrococcus horikoshii OT3
PhGBE
-
Pyrococcus horikoshii OT3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Pyrococcus horikoshii OT3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay az Pyrococcus horikoshii OT3

General Information

General Information Comment Organism
metabolism the glycogen branching enzyme catalyzes the formation of alpha-1,6-branching points during glycogenesis by cleaving alpha-1,4 bonds and making new alpha-1,6 bonds Pyrococcus horikoshii OT3