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Literature summary for 2.4.1.18 extracted from

  • Roussel, X.; Lancelon-Pin, C.; Vikso-Nielsen, A.; Rolland-Sabate, A.; Grimaud, F.; Potocki-Veronese, G.; Buleon, A.; Putaux, J.L.; DHulst, C.
    Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis (2012), Biochim. Biophys. Acta, 1830, 2167-2177.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Rhodothermus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for amylose is 0.7 mg/ml, 30°C, pH 7.0 Rhodothermus marinus
additional information
-
additional information enzyme reaction kinetics with different branched or unbranched alpha-glucans of controlled structure Rhodothermus marinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodothermus marinus the enzyme catalyzes starch branching by the cleavage of alpha(1->4) linkage and transfer in alpha(1->6) of the fragment in non-reducing position, but the enzyme also shows an additional alpha-4-glucanotransferase activity not described so far for a member of the GH13 family. The enzyme is able to transfer alpha(1->4)-linked-glucan in C4 position (instead of C6 position for the branching activity) of a glucan to create new alpha(1->4) linkages yielding to the elongation of linear chains subsequently used for further branching, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus Q93HU3
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Rhodothermus marinus Q93HU3 gene glgB
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108
-
30°C, pH 7.0 Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose potato type III amylose Rhodothermus marinus amylose containing alpha-1,6-glucosidic linkages
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?
additional information a minimal chain length of ten glucosyl units is required for the donor substrate to be recognized by Rhodothermus marinus branching enzyme that essentially produces branches with a degree of polymerization of 3-8. The enzyme preferentially creates new branches by intermolecular mechanism. Branched glucans define better substrates for the enzyme leading to the formation of hyper-branched particles of 30-70 nm in diameter, dextrins. The enzyme catalyzes an additional alpha-4-glucanotransferase activity Rhodothermus marinus ?
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?
additional information the enzyme catalyzes starch branching by the cleavage of alpha(1->4) linkage and transfer in alpha(1->6) of the fragment in non-reducing position, but the enzyme also shows an additional alpha-4-glucanotransferase activity not described so far for a member of the GH13 family. The enzyme is able to transfer alpha(1->4)-linked-glucan in C4 position (instead of C6 position for the branching activity) of a glucan to create new alpha(1->4) linkages yielding to the elongation of linear chains subsequently used for further branching, overview Rhodothermus marinus ?
-
?

Synonyms

Synonyms Comment Organism
RoBE
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Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodothermus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rhodothermus marinus

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family GH13 Rhodothermus marinus
metabolism glycogen and starch branching enzymes catalyze the formation of alpha(1->6) linkages in storage polysaccharides by rearrangement of preexisting alpha-glucans. This reaction occurs through the cleavage of alpha(1->4) linkage and transfer in alpha(1->6) of the fragment in non-reducing position. These enzymes define major elements that control the structure of both glycogen and starch Rhodothermus marinus