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Literature summary for 2.4.1.18 extracted from

  • Vu, N.T.; Shimada, H.; Kakuta, Y.; Nakashima, T.; Ida, H.; Omori, T.; Nishi, A.; Satoh, H.; Kimura, M.
    Biochemical and crystallographic characterization of the starch branching enzyme I (BEI) from Oryza sativa L (2008), Biosci. Biotechnol. Biochem., 72, 2858-2866.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method, 20°C Oryza sativa

Protein Variants

Protein Variants Comment Organism
D270A mutant with about 10% relative enzyme activity compared to wild-type enzyme Oryza sativa
D344A mutant without enzyme activity Oryza sativa
E399A mutant with about 5% relative enzyme activity compared to wild-type enzyme Oryza sativa
G468D mutant with about 95% relative enzyme activity compared to wild-type enzyme Oryza sativa
H275A mutant without enzyme activity Oryza sativa
H467A mutant without enzyme activity Oryza sativa
R342A mutant with about 15% relative enzyme activity compared to wild-type enzyme Oryza sativa
Y235A mutant with about 15% relative enzyme activity compared to wild-type enzyme Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q01401
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-sepharose chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
2.5 U/mg for amylopectin, 1 U = decrease of one absorbance unit at 660 nm at 30°C Oryza sativa
additional information
-
20.8 U/mg for amylose, 1 U = decrease of one absorbance unit at 660 nm at 30°C Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin assay at 30°C, reaction terminated by heating at 95°C for 5 min Oryza sativa ?
-
?
amylose assay at 30°C, reaction terminated by heating at 95°C for 5 min Oryza sativa ?
-
?

Synonyms

Synonyms Comment Organism
BEI
-
Oryza sativa
starch branching enzyme I
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Oryza sativa