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Literature summary for 2.4.1.18 extracted from
- A novel branching enzyme of the GH-57 family in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2006), J. Bacteriol., 188, 5915-5924.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Bl21-CodonPlus(DE3)-RIL and pET21a(+) | Thermococcus kodakarensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant TK1436 protein (amino acids [aa] 1 to 675) and a deletion derivative devoid of the C-terminal two-copy helix-hairpin-helix (HhH)2 motif (TK1436deltaH, aa 1 to 562) | Thermococcus kodakarensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78550 | - |
calculated molecular mass of the TK1436 protein | Thermococcus kodakarensis |
| 558000 | - |
TK1436deltaH protein, gel filtration on a Superdex 200 HR 10/30 column | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q5JDJ7 | TK1436 encodes a protein of 675 aa; hyperthermophilic archaeon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange column and hydrophobic column | Thermococcus kodakarensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the TK1436 protein shows rapid degradation within 2 to 3 days of storage at 4°C, while the TK1436deltaH protein is stable unter the same conditions | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TK1436 | encodes a branching enzyme from Thermococcus kodakaraensis belonging to the GH-57 family. The TK1436 protein contains neither alpha-galactosidase nor pullulanase activity. | Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
determined by iodine-staining assay | Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
determined by iodine-staining assay | Thermococcus kodakarensis |
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Release 2023.1 (January 2023)
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