BRENDA - Enzyme Database
show all sequences of 2.4.1.179

Characterization and membrane organization of beta 1-3- and beta 1-4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures

Holmes, E.H.; Arch. Biochem. Biophys. 270, 630-646 (1989)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Bovine liver non-specific transfer proteins
activation
Homo sapiens
CHAPSO
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
deoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
additional information
little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A
Homo sapiens
phosphatidylethanolamine
activation, less efficient than Triton CF-54
Homo sapiens
taurodeoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
Triton CF-54
activation, optimal at 0.1%
Homo sapiens
Triton X-100
activation, can replace Triton CF-54 with 75% less efficiency
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
lactotriaosylceramide
-
Homo sapiens
0.048
-
UDP-galactose
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
Golgi apparatus
-
Homo sapiens
5794
-
membrane
-
Homo sapiens
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mn2+ with 26% efficiency
Homo sapiens
Co2+
activation, can replace Mn2+ with 51% efficiency
Homo sapiens
Cu2+
activation, can replace Mn2+ with 15% efficiency
Homo sapiens
Mg2+
activation, can replace Mn2+ with 13% efficiency
Homo sapiens
Mn2+
requirement, 25 mM
Homo sapiens
additional information
no activation by Zn2+ or Cd2+
Homo sapiens
Ni2+
activation, can replace Mn2+ with 16% efficiency
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
Homo sapiens
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
from Colo 205 cell line; partial
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Homo sapiens
-
Colo-205 cell
colonic adenocarcino cell line
Homo sapiens
-
colonic adenocarcinoma cell
-
Homo sapiens
-
SW-403 cell
colonic adenocarcino cell line
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00077
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
-
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
substrate specificity
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
638380
Homo sapiens
?
UDP-galactose + lactotriaosylceramide
best substrate
638380
Homo sapiens
UDP + lactotetraosylceramide
-
638380
Homo sapiens
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
Hepes buffer
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.8
8.2
about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Bovine liver non-specific transfer proteins
activation
Homo sapiens
CHAPSO
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
deoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
additional information
little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A
Homo sapiens
phosphatidylethanolamine
activation, less efficient than Triton CF-54
Homo sapiens
taurodeoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
Triton CF-54
activation, optimal at 0.1%
Homo sapiens
Triton X-100
activation, can replace Triton CF-54 with 75% less efficiency
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
lactotriaosylceramide
-
Homo sapiens
0.048
-
UDP-galactose
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
Golgi apparatus
-
Homo sapiens
5794
-
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mn2+ with 26% efficiency
Homo sapiens
Co2+
activation, can replace Mn2+ with 51% efficiency
Homo sapiens
Cu2+
activation, can replace Mn2+ with 15% efficiency
Homo sapiens
Mg2+
activation, can replace Mn2+ with 13% efficiency
Homo sapiens
Mn2+
requirement, 25 mM
Homo sapiens
additional information
no activation by Zn2+ or Cd2+
Homo sapiens
Ni2+
activation, can replace Mn2+ with 16% efficiency
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
Homo sapiens
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
from Colo 205 cell line; partial
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Homo sapiens
-
Colo-205 cell
colonic adenocarcino cell line
Homo sapiens
-
colonic adenocarcinoma cell
-
Homo sapiens
-
SW-403 cell
colonic adenocarcino cell line
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00077
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
-
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
substrate specificity
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
638380
Homo sapiens
?
UDP-galactose + lactotriaosylceramide
best substrate
638380
Homo sapiens
UDP + lactotetraosylceramide
-
638380
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
Hepes buffer
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.8
8.2
about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer
Homo sapiens
Other publictions for EC 2.4.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
690818
Quintero
Calsenilin and CALP interact w ...
Homo sapiens
Biochem. J.
412
19-26
2008
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638381
Sherwood
Stable expression of a cDNA en ...
Homo sapiens
J. Cell. Biochem.
50
165-177
1992
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638377
Messer
Biosynthesis of marsupial milk ...
no activity in Mus musculus, Notamacropus eugenii
Biochim. Biophys. Acta
1077
79-85
1991
-
-
-
-
-
1
2
1
-
1
-
-
-
2
-
-
-
-
-
2
1
-
16
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
2
1
-
16
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
638379
Holmes
Preparative in vitro generatio ...
Homo sapiens
Arch. Biochem. Biophys.
274
14-25
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638380
Holmes
Characterization and membrane ...
Homo sapiens
Arch. Biochem. Biophys.
270
630-646
1989
8
-
-
-
-
-
-
2
3
7
-
1
-
1
-
-
1
-
-
4
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
2
3
7
-
1
-
-
-
1
-
4
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
638376
Bailly
Characterization and specific ...
Homo sapiens
Eur. J. Biochem.
173
417-422
1988
1
-
-
-
-
1
1
3
2
1
-
1
-
1
-
-
-
-
-
1
-
-
15
-
1
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
2
1
-
1
-
-
-
-
-
1
-
-
15
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
488980
Bailly
Identification of UDP-galactos ...
Homo sapiens
Biochem. Biophys. Res. Commun.
141
84-91
1986
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
1
-
-
1
-
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
1
-
1
-
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-