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Literature summary for 2.4.1.179 extracted from

  • Messer, M.; Nicholas, K.R.
    Biosynthesis of marsupial milk oligosaccharides: characterization and developmental changes of two galactosyltransferases in lactating mammary glands of the tammar wallaby, Macropus eugenii (1991), Biochim. Biophys. Acta, 1077, 79-85.
    View publication on PubMed
Search for more literature for 2.4.1.179

General Stability

General Stability Organism
Mn2+ enhances thermal stability Notamacropus eugenii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 97% inhibition at 30 mM Notamacropus eugenii
additional information bovine or gammar alpha-lactalbumin Notamacropus eugenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
46
-
 lactose
-
 Notamacropus eugenii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ absolute requirement Notamacropus eugenii

Organism

Organism UniProt Comment Textmining
no activity in Mus musculus
-
 lactating mammary gland
-
Notamacropus eugenii
-
 female tammar wallaby
-

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
 Notamacropus eugenii
-
additional information dramatic changes during lactation Notamacropus eugenii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02 0.032 from day 90 to day 225 post partum Notamacropus eugenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glucose is a very poor acceptor substrate Notamacropus eugenii ?
-
 ?
UDP-galactose + beta-D-galactose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-beta-D-galactose
-
 ?
UDP-galactose + beta-D-galactosyl-1,6-N-acetylglucosylamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,6-N-acetylglucosylamine
-
 ?
UDP-galactose + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
UDP-galactose + D-galactosyl-1,4-beta-D-glucose best substrate Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
UDP-galactose + D-galactosyl-1,4-beta-D-glucose i.e. lactose Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
-
 Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
 ?
UDP-galactose + lacto-N-tetraose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-lacto-N-tetraose
-
 ?
UDP-galactose + lactulose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-lactulose
-
 ?
UDP-galactose + melibiose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-melibiose
-
 ?
UDP-galactose + methyl-alpha-galactoside
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-methyl-alpha-galactoside
-
 ?
UDP-galactose + methyl-beta-galactoside
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-methyl-beta-galactoside
-
 ?
UDP-galactose + N-acetylgalactosamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetylgalactosamine
-
 ?
UDP-galactose + N-acetyllactosamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetyllactosamine
-
 ?
UDP-galactose + p-nitrophenyl-alpha-N-acetylgalactosylamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-galactosylaminyl-4-nitrophenyl
-
 ?
UDP-galactose + stachyose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-stachyose
-
 ?

Synonyms

Synonyms Comment Organism
UDP-galactose:lactose beta1,3-galactosyltransferase
-
 Notamacropus eugenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Notamacropus eugenii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36
-
 t1/2: 20 min Notamacropus eugenii
39
-
 30 min, complete inactivation, in the presence of Mn2+ 11% loss of activity within 60 min Notamacropus eugenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Notamacropus eugenii

pH Range

pH Minimum pH Maximum Comment Organism
5 8 about 75% of maximal activity at pH 5 and about half-maximal activity at pH 8.0 Notamacropus eugenii