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Literature summary for 2.4.1.177 extracted from

  • Landmann, C.; Fink, B.; Schwab, W.
    FaGT2: a multifunctional enzyme from strawberry (Fragaria x ananassa) fruits involved in the metabolism of natural and xenobiotic compounds (2007), Planta, 226, 417-428.
    View publication on PubMed
Search for more literature for 2.4.1.177

Cloned(Commentary)

Cloned (Comment) Organism
His-tag fusion protein expressed in Escherichia coli BL21(DE3)pLysS Fragaria x ananassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
 4-chlorocinnamic acid pH 8.0, 21°C Fragaria x ananassa
0.73
-
 Cinnamic acid pH 8.0, 21°C Fragaria x ananassa
0.93
-
 trans-3-(3-pyridyl)acrylic acid pH 8.0, 21°C Fragaria x ananassa
1.14
-
 2,4,5-Trichlorophenol pH 8.0, 21°C Fragaria x ananassa
1.33
-
 trans-3-(2-furyl)acrylic acid pH 8.0, 21°C Fragaria x ananassa
2.74
-
 anthranilic acid pH 8.0, 21°C Fragaria x ananassa
2.75
-
 nicotinic acid pH 8.0, 21°C Fragaria x ananassa
2.85
-
 3,5-dichloro-4-hydroxybenzoic acid pH 8.0, 21°C Fragaria x ananassa
3.23
-
 sorbic acid pH 8.0, 21°C Fragaria x ananassa
5.76
-
 trans-2-hexenoic acid pH 8.0, 21°C Fragaria x ananassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, highest activity at 25-50 mM Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Fragaria x ananassa catalyzes the formation of cinnamic acid and p-coumaric acid glucose esters during strawberry fruit ripening, experimental results suggest that FaGT2 is involved in the detoxification of xenobiotics in accordance to its induction by oxidative stress ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Fragaria x ananassa

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-bis(4-chlorophenyl) acetic acid + UDP-glucose
-
 Fragaria x ananassa ?
-
 ?
2,4,5-trichlorophenol + UDP-glucose 225% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
2,4-dichlorophenoxyacetic acid + UDP-glucose
-
 Fragaria x ananassa ?
-
 ?
2,5-dimethyl-4-hydroxy-3[2H]-furanone + UDP-glucose
-
 Fragaria x ananassa ?
-
 ?
3,5-dichloro-4-hydroxybenzoic acid + UDP-glucose 102% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
4-chlorocinnamic acid + UDP-glucose 56% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
anthranilic acid + UDP-glucose 11% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
cinnamic acid + UDP-glucose 100% of activity Fragaria x ananassa UDP + cinnamoyl-beta-D-glucoside
-
 ?
additional information catalyzes the formation of cinnamic acid and p-coumaric acid glucose esters during strawberry fruit ripening, experimental results suggest that FaGT2 is involved in the detoxification of xenobiotics in accordance to its induction by oxidative stress Fragaria x ananassa ?
-
 ?
additional information most of the substrates also tested in plants by injecting the substance into fresh ripe strawberries Fragaria x ananassa ?
-
 ?
nicotinic acid + UDP-glucose 5% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
sorbic acid + UDP-glucose 126% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
trans-2-hexenoic acid + UDP-glucose 12% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
trans-3-(2-furyl)acrylic acid + UDP-glucose 94% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?
trans-3-(3-pyridyl)acrylic acid + UDP-glucose 9% of the activity with cinnamic acid Fragaria x ananassa ?
-
 ?

Synonyms

Synonyms Comment Organism
FaGT2
-
 Fragaria x ananassa