Cloned (Comment) | Organism |
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gene WsSGTL1, ectopic overexpression in Withania somnifera using Agrobacterium tumefaciens transfection method, quantitative real-time PCR enzyme expression analysis. Overexpression of WsSGTL1 gene which is responsible for increase in glycosylated withanolide and sterols, and confers better growth and tolerance to both biotic and abiotic stresses | Withania somnifera |
Protein Variants | Comment | Organism |
---|---|---|
additional information | WsGTL1 transgenic plants overexpressing the enzyme display a number of alterations at phenotypic and metabolic level in comparison to wild-type plants which include: (1) early and enhanced growth with leaf expansion and increase in number of stomata, (2) increased production of glycowithanolide (majorly withanoside V) and campesterol, stigmasterol and sitosterol in glycosylated forms with reduced accumulation of withanolides (withaferin A, withanolide A and withanone), (3) tolerance towards biotic stress, improved survival capacity under abiotic stress (cold stress), (4) enhanced recovery capacity after cold stress, as indicated by better photosynthesis performance, chlorophyll, anthocyanin content and better quenching regulation of PSI and PSII, phenotype overview | Withania somnifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | - |
gene WsSGTL1 | - |
Withania somnifera NMITLI-101 | - |
gene WsSGTL1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Withania somnifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols | Withania somnifera | ? | - |
? | |
additional information | the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols | Withania somnifera NMITLI-101 | ? | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | ectopic enzyme overexpression in Withania somnifera promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses. Glycosylation not only stabilizes the products but also alters their physiological activities and governs intracellular distribution | Withania somnifera |