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Literature summary for 2.4.1.173 extracted from
- Molecular cloning and characterization of one member of 3beta-hydroxy sterol glucosyltransferase gene family in Withania somnifera (2007), Arch. Biochem. Biophys., 460, 48-55.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain DH5alpha | Arabidopsis thaliana |
| the open reading frame of SGTL1 is expressed in Escherichia coli strain DH5alpha | Withania somnifera |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
solasodine | - |
Arabidopsis thaliana |  |
| 0.004 | - |
ergosterol | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.0045 | - |
brassicasterol | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.006 | - |
pregnenolone | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.007 | - |
3beta-hydroxy-pregna-5,16-dien-20-one | - |
Arabidopsis thaliana | |
| 0.007 | - |
solasodine | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.008 | - |
pregnenolone | - |
Arabidopsis thaliana | |
| 0.008 | - |
UDP-glucose | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.01 | - |
3beta-hydroxy-16,17alpha-epoxypregnenolone | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.01 | - |
3beta-hydroxy-pregna-5,16-dien-one | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.017 | - |
3beta-hydroxy-16,17-alpha-epoxypregnenolene | - |
Arabidopsis thaliana | |
| 0.025 | - |
UDP-glucose | - |
Arabidopsis thaliana | |
| 0.025 | - |
transandrosterone | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.027 | - |
stigmasterol | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.03 | - |
brassicasterol | - |
Arabidopsis thaliana | |
| 0.035 | - |
ergosterol | - |
Arabidopsis thaliana | |
| 0.04 | - |
dehydroepiandrosterone | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.04 | - |
beta-sitosterol | activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera | |
| 0.043 | - |
dehydroepiandrosterone | - |
Arabidopsis thaliana | |
| 0.043 | - |
stigmasterol | - |
Arabidopsis thaliana | |
| 0.056 | - |
cholesterol | - |
Arabidopsis thaliana | |
| 0.059 | - |
beta-sitosterol | - |
Arabidopsis thaliana | |
| 0.059 | - |
transandrosterone | - |
Arabidopsis thaliana | |
| 0.063 | - |
5alpha-Cholestan-3beta-ol | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
SDS-PAGE | Withania somnifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Withania somnifera | Q2I015 | the incubation of leaf discs in the presence of salicylic acid results in transient accumulation of SGTL1 transcript. It increases gradually to reach the maxima of 2.15fold at 12 h followed by a decline. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration and ion exchange column chromatography | Arabidopsis thaliana |
| partially purified by gel filtration chromatography, resutlts in 3.73fold purification | Withania somnifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | from 4 weeks old plants, young and mature, 2.21fold higher enzyme level than in the stem, SGTL1 transcript accumulates at a higher level in mature leaves (2.41fold) than in the young leaves and seedlings | Withania somnifera | - |
| root | from 4 weeks old plants, 3.13fold higher expression of the enzyme than in the stem | Withania somnifera | - |
| seedling | lower expression of the enzyme than in mature leaves | Withania somnifera | - |
| stem | from 4 weeks old plants, expressend in significantly lower level than in leaves and roots | Withania somnifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the activity obtained with dehydroepiandrosterone is 100%. 3-beta-hydroxy-pregna-5,16-dien-one, transandrosterone, 3-beta-hydroxy-16, 17-alpha-epoxy-pregnenolone, pregnenolone, stigmasterol, beta-sitosterol, ergosterol, brassicasterol and solasodine give activities with the enzyme SGTL1. 5-alpha-cholestan-3-beta-ol and cholesterol show no activity with the enzyme. Activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C | Withania somnifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose | - |
Arabidopsis thaliana | 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP | - |
? | |
| 3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose | - |
Arabidopsis thaliana | UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside | - |
? | |
| 5alpha-cholestan-3beta-ol + UDP-glucose | - |
Arabidopsis thaliana | UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside | - |
? | |
| beta-sitosterol + UDP-glucose | - |
Arabidopsis thaliana | UDP + beta-sitosterol 3-beta-D-glucoside | - |
? | |
| brassicasterol + UDP-glucose | - |
Arabidopsis thaliana | UDP + brassicasterol 3-beta-D-glucoside | - |
? | |
| cholesterol + UDP-glucose | - |
Arabidopsis thaliana | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| dehydroepiandrosterone + UDP-glucose | - |
Arabidopsis thaliana | UDP + dehydroepiandrosterone 3-beta-D-glucoside | - |
? | |
| ergosterol + UDP-glucose | - |
Arabidopsis thaliana | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| additional information | the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor. | Withania somnifera | ? | - |
? | |
| additional information | the enzyme only shows activity with sterols having hydroxyl group at C-3 position. Utilises only UDP-glucose, UDP-galactose cannot serve as the sugar donor. | Arabidopsis thaliana | ? | - |
? | |
| pregnenolene + UDP-glucose | - |
Arabidopsis thaliana | UDP + pregnenolone 3-beta-D-glucoside | - |
? | |
| solasodine + UDP-glucose | - |
Arabidopsis thaliana | UDP + solasodine 3-beta-D-glucoside | - |
? | |
| stigmasterol + UDP-glucose | - |
Arabidopsis thaliana | UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone | - |
Withania somnifera | ? | - |
? | |
| UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone | - |
Arabidopsis thaliana | UDP + 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + 3beta-hydroxy-pregna-5,16-dien-one | - |
Withania somnifera | UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside | - |
? | |
| UDP-glucose + beta-sitosterol | - |
Withania somnifera | UDP + beta-sitosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + brassicasterol | - |
Withania somnifera | UDP + brassicasterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + dehydroepiandrosterone | - |
Withania somnifera | UDP + dehydroepiandrosterone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Withania somnifera | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + pregnenolone | - |
Arabidopsis thaliana | UDP + pregnenolone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + pregnenolone | - |
Withania somnifera | UDP + pregnenolone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + solasodine | - |
Withania somnifera | UDP + solasodine 3-beta-D-glucoside | - |
? | |
| UDP-glucose + stigmasterol | - |
Withania somnifera | UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + transandrosterone | - |
Arabidopsis thaliana | UDP + transandrosterone 3beta-D-glucoside | - |
? | |
| UDP-glucose + transandrosterone | - |
Withania somnifera | UDP + transandrosterone 3beta-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtSGT | sterol glucosyltransferase in Arabidopsos thaliana | Arabidopsis thaliana |
| SGT | sterol glucosyltransferase | Withania somnifera |
| SGTL1 | - |
Withania somnifera |
| SGTL1 | gene encoding a member of the sterol glucosyltransferase in Withania somnifera | Withania somnifera |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a decrease in the transcript level is observed within 15 min when the plants are shifted from 25 to 42°C | Withania somnifera |
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