BRENDA - Enzyme Database
show all sequences of 2.4.1.173

UDPG:sterol glucosyltransferase in etiolated pea seedlings

Fang, T.Y.; Baisted, D.J.; Phytochemistry 15, 273-278 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
slightly stimulation at low concentration
Pisum sativum
phosphatidylcholine
33% increase of activity
Pisum sativum
phosphatidylethanolamine
increases 125% of activity
Pisum sativum
phosphatidylserine
33% increase of activity
Pisum sativum
phospholipase
treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
Pisum sativum
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
inhibition at 1-3 mM but slightly stimulation at low concentration
Pisum sativum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
etioplast
-
Pisum sativum
9513
-
Golgi apparatus
-
Pisum sativum
5794
-
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximal stimulation at 13 mM
Pisum sativum
additional information
no metal ion requirement
Pisum sativum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
pea
-
Purification (Commentary)
Commentary
Organism
partial purification by differential centrifugation
Pisum sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
etiolated
Pisum sativum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + sitosterol
-
637775
Pisum sativum
UDP + sitosterol 3-beta-D-glucoside
-
637775
Pisum sativum
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pisum sativum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
Tris-HCl 25 mM
Pisum sativum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
slightly stimulation at low concentration
Pisum sativum
phosphatidylcholine
33% increase of activity
Pisum sativum
phosphatidylethanolamine
increases 125% of activity
Pisum sativum
phosphatidylserine
33% increase of activity
Pisum sativum
phospholipase
treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
Pisum sativum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
inhibition at 1-3 mM but slightly stimulation at low concentration
Pisum sativum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
etioplast
-
Pisum sativum
9513
-
Golgi apparatus
-
Pisum sativum
5794
-
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximal stimulation at 13 mM
Pisum sativum
additional information
no metal ion requirement
Pisum sativum
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification by differential centrifugation
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
etiolated
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + sitosterol
-
637775
Pisum sativum
UDP + sitosterol 3-beta-D-glucoside
-
637775
Pisum sativum
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pisum sativum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
Tris-HCl 25 mM
Pisum sativum
Other publictions for EC 2.4.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736981
Saema
Ectopic overexpression of WsSG ...
Withania somnifera, Withania somnifera NMITLI-101
Plant Cell Rep.
35
195-211
2016
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1
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1
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5
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1
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2
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1
1
-
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-
736570
Stucky
Functional diversification of ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Exp. Bot.
66
189-201
2015
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1
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1
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12
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9
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1
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18
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12
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3
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18
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3
7
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-
-
737041
Li
Distinct biochemical activitie ...
Gossypium hirsutum, Gossypium hirsutum Huamian99
Plant Sci.
219-220
1-8
2014
2
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1
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3
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5
1
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4
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5
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1
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1
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4
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2
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4
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2
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4
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2
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1
2
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736786
Malik
Sterol 3beta-glucosyltransfera ...
Debaryomyces hansenii, Debaryomyces hansenii CBS 767, Kluyveromyces lactis, Kluyveromyces lactis DSM 70799, Komagataella pastoris, Komagataella pastoris GS115, Meyerozyma guilliermondii, Meyerozyma guilliermondii DSM 6381, Ogataea angusta, Ogataea angusta DSM 70277, Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Mol. Biosyst.
9
2816-2822
2013
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6
-
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-
3
-
6
3
-
-
14
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6
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-
-
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60
3
6
-
-
3
6
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6
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-
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3
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6
3
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6
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-
-
60
3
6
-
-
3
6
-
-
-
-
-
-
-
3
3
723149
Chaturvedi
Sterol glycosyltransferases-id ...
Saccharomyces cerevisiae
Mol. Biol. Rep.
39
9755-9764
2012
-
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1
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-
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2
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1
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1
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4
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1
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1
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1
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1
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1
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2
2
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722093
Akiyama
-
A possible mechanism of choles ...
Homo sapiens
Cytologia
76
19-25
2011
-
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-
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1
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1
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1
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1
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1
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2
2
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706211
Asakura
Atg26-mediated pexophagy is re ...
Colletotrichum orbiculare
Plant Cell
21
1291-1304
2009
-
-
-
-
-
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6
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1
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1
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2
2
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-
706323
DeBolt
Mutations in UDP-glucose:stero ...
Arabidopsis thaliana
Plant Physiol.
151
78-87
2009
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1
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1
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5
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7
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7
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690224
Potocka
Metabolism of conjugated stero ...
Solanum melongena
Acta Biochim. Pol.
55
127-134
2008
3
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-
17
2
1
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1
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3
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1
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1
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24
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4
3
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4
17
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2
1
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1
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1
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1
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24
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671624
Sharma
Molecular cloning and characte ...
Arabidopsis thaliana, Withania somnifera
Arch. Biochem. Biophys.
460
48-55
2007
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2
-
-
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24
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1
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6
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2
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4
1
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22
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1
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2
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24
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1
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2
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4
1
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22
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1
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-
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-
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672427
Madina
Purification and physico-kinet ...
Withania somnifera
Biochim. Biophys. Acta
1774
392-402
2007
1
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11
8
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1
1
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3
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1
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1
1
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8
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1
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8
1
1
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1
4
1
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4
11
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8
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1
1
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1
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1
1
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8
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1
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8
1
1
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1
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690681
Cao
Atg26 is not involved in autop ...
Saccharomyces cerevisiae
Autophagy
3
17-20
2007
-
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-
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1
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4
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1
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658411
Stasyk
Sterol glucosyltransferases ha ...
Komagataella pastoris, Yarrowia lipolytica
Cell Biol. Int.
27
947-952
2003
-
-
-
-
-
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2
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5
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2
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658544
Oku
Peroxisome degradation require ...
Komagataella pastoris, Komagataella pastoris GS115
EMBO J.
22
3231-3241
2003
-
-
1
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1
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2
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1
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6
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1
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1
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1
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1
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660084
Idnurm
-
Characterisation of neutral tr ...
Leptosphaeria maculans
Physiol. Mol. Plant Pathol.
62
305-313
2003
-
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1
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1
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660164
Kim
Identification of a hard surfa ...
Colletotrichum gloeosporioides
Plant J.
30
177-187
2002
-
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1
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1
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1
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1
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1
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637784
Warnecke
Cloning and functional express ...
Avena sativa, Avena sativa ugt80A1, Candida albicans, Dictyostelium discoideum, Dictyostelium discoideum ugt52, Komagataella pastoris, Komagataella pastoris GS115, Saccharomyces cerevisiae
J. Biol. Chem.
274
13048-13059
1999
-
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4
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1
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4
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20
-
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40
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4
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4
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1
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4
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-
40
-
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4
-
-
-
-
-
-
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637783
Warnecke
UDP-glucose:sterol glucosyltra ...
Arabidopsis thaliana, Avena sativa, Avena sativa ugt80A1
Plant Mol. Biol.
35
597-603
1997
-
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2
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2
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8
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5
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3
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2
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2
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2
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5
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3
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2
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2
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-
637782
Warnecke
Purification of a membrane-bou ...
Avena sativa
Plant Physiol.
105
1067-1073
1994
-
-
-
-
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3
2
1
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1
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2
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1
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1
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5
-
1
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1
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1
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3
1
2
1
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1
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1
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1
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5
-
1
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-
-
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1
-
-
-
-
-
-
-
637781
Ullmann
UDP-glucose sterol beta-D-gluc ...
Zea mays
Biochimie
75
713-723
1993
-
-
-
-
-
-
5
2
1
-
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4
-
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1
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1
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1
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1
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5
1
2
1
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1
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1
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1
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-
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-
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637785
Bergenstraahle
-
Effects of ethephon and norbor ...
Solanum tuberosum
Physiol. Plant.
89
301-308
1993
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
637773
Paczkowski
-
Steroid-specific glucosyltrans ...
Asparagus setaceus
Phytochemistry
29
63-70
1990
3
-
-
-
-
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4
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637767
Murakami-Murofushi
Expression of UDP-glucose: por ...
Physarum polycephalum
Biochim. Biophys. Acta
992
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1989
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637772
Ury
Phospholipid dependence of pla ...
Zea mays
Plant Physiol.
91
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1989
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488870
Kalinowska
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Subcellular localization of UD ...
Avena sativa
Phytochemistry
26
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1987
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637768
Ullmann
Regulation by phospholipids an ...
Zea mays
Plant Physiol.
85
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1987
3
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208429
Kalinowska
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Modulation of activities of st ...
Sinapis alba
Phytochemistry
25
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1986
5
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488869
Kalinowska
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Enzymatic synthesis of nuatige ...
Avena sativa
Phytochemistry
25
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1986
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637778
Bouvier-Nave
-
Phospholipid-dependence of pla ...
Zea mays
Plant Sci. Lett.
36
19-27
1984
5
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637780
Ullmann
-
Phospholipid-dependence of pla ...
Zea mays
Plant Sci. Lett.
36
29-36
1984
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637779
Quantin-Martenot
-
Activation of etiolated maize ...
Zea mays
Plant Sci. Lett.
29
305-314
1983
3
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1
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637770
Yoshikawa
-
Purification and properties of ...
Digitalis purpurea
Phytochemistry
18
239-241
1979
3
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637771
Wojciechowski
Specificity of sterol-glucosyl ...
Physarum polycephalum, Sinapis alba
Biochim. Biophys. Acta
570
363-370
1979
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2
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637776
Staver
Uridine diphosphate glucose-st ...
Pisum sativum
Biochem. J.
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1978
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637766
Wojciechowski
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Enzymatic synthesis of steryl ...
Physarum polycephalum
Phytochemistry
16
911-914
1977
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637775
Fang
-
UDPG:sterol glucosyltransferas ...
Pisum sativum
Phytochemistry
15
273-278
1976
5
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Forsee
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Solubilization of a particulat ...
Gossypium hirsutum
Arch. Biochem. Biophys.
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1974
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Esders
Occurrence of a uridine diphos ...
Pseudohyphozyma bogoriensis
J. Biol. Chem.
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1972
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