Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activated by | Digitalis purpurea | |
DIECA | activated by | Digitalis purpurea | |
EDTA | activated by | Digitalis purpurea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NH4+ | inhibited by | Digitalis purpurea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Digitalis purpurea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | inhibited by | Digitalis purpurea | |
Zn2+ | inhibited by | Digitalis purpurea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Digitalis purpurea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B | Digitalis purpurea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | leaf cell culture | Digitalis purpurea | - |
leaf | crude extracts have 2-5 times lower activity than cell cultures | Digitalis purpurea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | almost no activity with androsterone, digitoxigenin, digitoxin and testosterone | Digitalis purpurea | ? | - |
? | |
UDP-glucose + cholesterol | best substrate | Digitalis purpurea | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + epiandrosterone | best substrate | Digitalis purpurea | UDP + epiandrosterone 3-beta-glucoside | - |
? | |
UDP-glucose + pregnenolone | 57% of the activity with epiandrosterone | Digitalis purpurea | UDP + pregnenolone 3-beta-glucoside | - |
? | |
UDP-glucose + sitosterol | 88% of the activity with epiandrosterone | Digitalis purpurea | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | 122% of the activity with epiandrosterone, highest specificity | Digitalis purpurea | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Digitalis purpurea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Digitalis purpurea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl 0.1 M, EDTA 10 mM, 2-mercaptoethanol 1 mM, sodium-deoxycholate 0.1% | Digitalis purpurea |