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Literature summary for 2.4.1.17 extracted from
- Mapping the UDP-glucuronic acid binding site in UDP-glucuronosyltransferase-1A10 by homology-based modeling: confirmation with biochemical evidence (2008), Biochemistry, 47, 7385-7392.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged isozymes UGT1A10 or UGT1A7, and of mutant constructurs in COS-1 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of mutants K314R/Q/A/E/G and K404R/E, that have null activities or 2.7-fold higher/50% less activity, respectively, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP-glucose | competitive inhibition, predicted binding sites in isozyme UGT1A10 are sites N292, K314, K315, and K404, overview | Homo sapiens |  |
| UDP-hexanol amine | competitive inhibition, predicted binding sites in isozyme UGT1A10 are sites N292, K314, K315, and K404 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucuronate + acceptor | Homo sapiens | - |
UDP + acceptor beta-D-glucuronoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9HAW8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant His-tagged isozymes UGT1A10 or UGT1A7 about 200fold from the 60000 x g supernatant by nickel affinity chromatography, binding analysis on affinity ligand 5-azidouridine-diphosphoglucuronic acid, which possesses high- and low-affinity binding sites, determination and analysis, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | determination of UDP-glucuronate binding sites in isozymes UGT1A10 and UGT1A7 using inhibitor binding and peptide mapping, overview | Homo sapiens | ? | - |
? | |
| UDP-D-glucuronate + acceptor | - |
Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + mycophenolic acid | K314 and K404m are determined binding sites required for UDP-glcA binding to isozyme UGT1A10, overview | Homo sapiens | UDP + mycophenolic acid beta-D-glucuronoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology-based computer modeling of isozyme UGT1A10, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucuronosyltransferase | - |
Homo sapiens |
| UDP-glucuronosyltransferase-1A10 | - |
Homo sapiens |
| UGT1A10 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7.6 | assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics analysis, overview | Homo sapiens |
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Release 2023.1 (January 2023)
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