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Literature summary for 2.4.1.17 extracted from

  • Kakuda, S.; Oka, S.; Kawasaki, T.
    Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli (2004), Protein Expr. Purif., 35, 111-119.
    View publication on PubMed
Search for more literature for 2.4.1.17

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylinositol highly required for activity of GlcAT-P and GlcAT-S with paraglobosid and other glycolipids Homo sapiens
sphingomyelin almost indispensable activator of GlcAT-P as to glycoprotein acceptors Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of FLAG-tagged enzymes GlcAT-P and GlcAT-S in Escherichia coli strain BL21 as soluble proteins Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
asialo-orosomucoid inhibits activity with paragloboside Homo sapiens
lacto-N-biose 22% inhibition of recombinant GlcAT-P and 77.3% of recombinant GlcAT-S at 5 mM Homo sapiens
lactose 24.5% inhibition of recombinant GlcAT-S at 5 mM, no inhibition of recombinant GlcAT-P Homo sapiens
N-acetyllactosamine 87.4% inhibition of recombinant GlcAT-P and 83.7% of recombinant GlcAT-S at 5 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme are involved in biosynthesis of HNK-1 carbohydrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble recombinant FLAG-tagged enzymes GlcAT-P and GlcAT-S, 1233fold and 7511fold, respectively, from Escherichia coli by affinity chromatographic steps Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
nervous system
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 purified recombinant GlcAT-S with substrate asialo-orosomucoid Homo sapiens
1.11
-
 purified recombinant GlcAT-P with substrate asialo-orosomucoid Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme are involved in biosynthesis of HNK-1 carbohydrate Homo sapiens ?
-
 ?
additional information the recombinant GlcAT-P is highly specific for terminal type II structure, Galbeta1-4GlcNAc, while the recombinant GlcAT-S recognizes type I, Galbeta1-3GlcNAc, and type II structures Homo sapiens ?
-
 ?
UDP-glucuronate + asialo-orosomucoid acceptor is a glycoprotein, preferred substrate Homo sapiens UDP + asialo-orosomucoid beta-D-glucuronide
-
 ?
UDP-glucuronate + paragloboside acceptor is a glycolipid, activity requires phosphatidylinositol Homo sapiens UDP + paragloboside beta-D-glucuronide
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GlcAT-P
-
 Homo sapiens
GlcAT-S
-
 Homo sapiens
glucuronyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens