Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analyis of wild-type isozyme UGT1A10, expression of wild-type and mutant isozymes UGT1A10 in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M59I | mutation in isozyme UGT1A10, site-directed mutagenesis, activity similar to the wild-type enzyme | Homo sapiens |
T202I | mutation in isozyme UGT1A10, site-directed mutagenesis, about 50% reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00192 | - |
17beta-estradiol | recombinant mutant M59I, pH 7.5, 37°C | Homo sapiens | |
0.00233 | - |
17beta-estradiol | recombinant mutant T202I, pH 7.5, 37°C | Homo sapiens | |
0.00234 | - |
17beta-estradiol | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
0.00418 | - |
7-hydroxy-4-trifluoromethylcoumarin | recombinant mutant M59I, pH 7.5, 37°C | Homo sapiens | |
0.00491 | - |
7-hydroxy-4-trifluoromethylcoumarin | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
0.00537 | - |
7-hydroxy-4-trifluoromethylcoumarin | recombinant mutant T202I, pH 7.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAW8 | isozyme UGT1A10; isozyme UGT1A10 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | isozyme UGT1A10 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + 17beta-estradiol | - |
Homo sapiens | UDP + 17beta-estradiol 3-O-glucuronide | - |
? | |
UDP-glucuronate + 7-hydroxy-4-trifluoromethylcoumarin | - |
Homo sapiens | UDP + 7-hydroxy-4-trifluoromethylcoumarin beta-D-glucuronide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase 1A10 | - |
Homo sapiens |
UGT1A10 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |