Any feedback?
Literature summary for 2.4.1.17 extracted from
- 4-Nitrophenol UDPglucuronyltransferase (rat liver) (1981), Methods Enzymol., 77, 169-177.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Alkyl ketones | activation | Rattus norvegicus |  |
| Diethylnitrosamine | 2fold to 3fold activation towards 2-aminophenol | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Lubrol 12A9 | - |
Rattus norvegicus | |
| additional information | not inhibitory: N-ethylmaleimide | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 to 20 mM, 30% increased activity | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, SDS-polyacrylamide gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar strain | - |
| Rattus norvegicus Wistar | - |
Wistar strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| several methods including chromatofocusing, combined with anion exchange chromatography and/or affinity chromatography of UDP-hexanolamine Sepharose | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% w/v glycerol, complete loss of activity | Rattus norvegicus |
| 0°C, half-life 18-20 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthol + UDP-glucuronate | - |
Rattus norvegicus | naphth-1-yl-beta-D-glucuronide + UDP | - |
? | |
| 1-naphthol + UDP-glucuronate | - |
Rattus norvegicus Wistar | naphth-1-yl-beta-D-glucuronide + UDP | - |
? | |
| 4-nitrophenol + UDP-glucuronate | - |
Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide + UDP | - |
? | |
| 4-nitrophenol + UDP-glucuronate | - |
Rattus norvegicus Wistar | 4-nitrophenyl beta-D-glucuronide + UDP | - |
? | |
| morphine + UDP-glucuronate | - |
Rattus norvegicus | UDP + morphine 3,6-diglucuronide | - |
? | |
| morphine + UDP-glucuronate | - |
Rattus norvegicus Wistar | UDP + morphine 3,6-diglucuronide | - |
? | |
| testosterone + UDPglucuronate | - |
Rattus norvegicus | UDP + testosterone O-glucuronide | - |
? | |
| testosterone + UDPglucuronate | - |
Rattus norvegicus Wistar | UDP + testosterone O-glucuronide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-polyacrylamide gel electrophoresis | Rattus norvegicus |
| ? | additional values for rat isozymes ranging from 51000 to 59000 | Rattus norvegicus |
| ? | all values determined by SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
inactivation in 15 min | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
