Cloned (Comment) | Organism |
---|---|
comparative sequence analysis, cloning and expression of the enzyme's transmembrane segment ArCS1_E22TM, recombinant expression of the His-tagged domain in Dictyostelium discoideum, the corresponding transmembrane protein ArCS1_E22TM accumulates in membrane fractions of the expression host Dictyostelium discoideum, recombinant expression of clone EIIIab containing the ArCS1_E22 cDNA fragment in Escherichia coli strain BL21(DE3) | Atrina rigida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | assembly mechanism of the chitin synthase Ar-CS1 via its extracellular domain ArCS1_E22 | Atrina rigida | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Atrina rigida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
size analysis of proteins purified at pH 4.7, pH 6.5, and pH 7.5 by using gel filtration, native PAGE, and dynamic light scattering | Atrina rigida |
13500 | - |
x * 21500, recombinant transmembrane segment ArCS1_E22, x * 13500, recombinant transmembrane segment ArCS1_E22, SDS-PAGE | Atrina rigida |
21500 | - |
x * 21500, recombinant transmembrane segment ArCS1_E22, x * 13500, recombinant transmembrane segment ArCS1_E22, SDS-PAGE | Atrina rigida |
40000 | 66000 | purified recombinant enzyme, gel filtration | Atrina rigida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atrina rigida | Q288C6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged transmembrane protein ArCS1_E22TM from Dictyostelium discoideum by nickel affinity chromatography, soluble recombinant ArCS1_E22 proteins can be purified as monomers only at basic pH of 9.0 | Atrina rigida |
Subunits | Comment | Organism |
---|---|---|
? | x * 21500, recombinant transmembrane segment ArCS1_E22, x * 13500, recombinant transmembrane segment ArCS1_E22, SDS-PAGE | Atrina rigida |
More | assembly mechanism of the chitin synthase Ar-CS1 via its extracellular domain ArCS1_E22. ArCS1_E22 forms regular nanostructures on cationic substrates, atomic force microscopy | Atrina rigida |
Synonyms | Comment | Organism |
---|---|---|
Ar-CS1 | - |
Atrina rigida |
CHS | - |
Atrina rigida |
General Information | Comment | Organism |
---|---|---|
physiological function | the extracellular domain ArCS1_E22 is involved in regulating the multiple enzyme activities of Ar-CS1 such as chitin synthesis and myosin movements by interaction with mineral surfaces and eventually by protein assembly. The protein complexes can locally probe the status of mineralization according to pH unless ions and pCO2 are balanced with suitable buffer substances. The intact enzyme can act as a force sensor. The shell formation is coordinated physiologically with precise adjustment of cellular activities to the structure, topography and stiffness at the mineralizing interface | Atrina rigida |