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Literature summary for 2.4.1.16 extracted from

  • Zhuo, W.; Chu, F.; Kong, L.; Tao, H.; Sima, Y.; Xu, S.
    Chitin synthase B: a midgut-specific gene induced by insect hormones and involved in food intake in Bombyx mori larvae (2014), Arch. Insect Biochem. Physiol., 85, 36-47.
    View publication on PubMed

Application

Application Comment Organism
agriculture the enzyme is a specific target of insecticides Bombyx mori

Cloned(Commentary)

Cloned (Comment) Organism
gene BmChsB, DNA and amino acid sequence determination and analysis, seqeunce comparisons, quantitative expression analysis and expression profile Bombyx mori

Protein Variants

Protein Variants Comment Organism
additional information gene BmChsB RNA interfering analysis, overview Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
nikkomycin Z exposure to nikkomycin Z, a CHS inhibitor, reduces the amount of chitin in the peritrophic membrane of molted larvae Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
123884
-
x * 123884, sequence calculation Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori Q9GV28 gene BmChsB
-

Source Tissue

Source Tissue Comment Organism Textmining
integument lower enzyme level Bombyx mori
-
larva
-
Bombyx mori
-
midgut
-
Bombyx mori
-
additional information gene BmChsB expression profiles in the midgut during molting and the mulberry-leaf ingestion processes Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
Bombyx mori UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
?

Subunits

Subunits Comment Organism
? x * 123884, sequence calculation Bombyx mori

Synonyms

Synonyms Comment Organism
CHS B
-
Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori sequence calculation
-
7.29

Expression

Organism Comment Expression
Bombyx mori exogenous 20-hydroxyecdysone and methoprene, a juvenile hormone analogue, significantly upregulate the expression of BmChsB when the levels of endogenous molting hormone are low and the levels of endogenous juvenile hormone are high immediately after molting. When levels of endogenous molting hormone are high and those of endogenous juvenile hormone are low during the molting stage, exogenous 20-hydroxyecdyson does not upregulate BmChsB expression and exogenous methoprene upregulates it negligibly. When the endogenous hormone levels are low during the mulberry-leaf intake process, BmChsB expression is upregulated by exogenous methoprene up

General Information

General Information Comment Organism
malfunction exposure to nikkomycin Z, a CHS inhibitor, reduces the amount of chitin in the peritrophic membrane of molted larvae Bombyx mori
physiological function chitin synthase is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. Expression of BmChsB is regulated by insect hormones, and directly affects the chitin-synthesis-dependent form of the peritrophic membrane and protects the food intake and molting process of silkworm larvae. Gene BmChsB is a midgut-specific gene induced by insect hormones and involved in protecting the food intake of Bombyx mori larvae Bombyx mori