Literature summary for 2.4.1.16 extracted from

Martinez-Rucobo, F.W.; Eckhardt-Strelau, L.; Terwisscha van Scheltinga, A.C.
Yeast chitin synthase 2 activity is modulated by proteolysis and phosphorylation (2009), Biochem. J., 417, 547-554.

Search for more literature for 2.4.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	Chs2 is hyperactivated by a soluble yeast protease, addition of leupeptin, a serine and cysteine protease inhibitor, almost completely abolishes the stimulatory effect, while it does not affect the activity of Chs2 or Chs2DELTAN222 by itself	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
high level expression of wild-type Chs2 and a mutant Chs2DELTAN222 lacking the N-terminal region in Escherichia coli strain C41 and in Pichia pastoris strain SMD1163 membranes in an active form, the latter system results in higher enzyme activity, overview	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a Chs2 mutant Chs2DELTAN222 lacking the N-terminal region	Saccharomyces cerevisiae

General Stability

General Stability	Organism
wild-type isozyme Chs2 has a half-life of 25 min	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	complete inhibition of wild-type and mutant enzymes	Saccharomyces cerevisiae
Co2+	inhibits the wild-type Chs2 and mutant Chs2DELTAN222	Saccharomyces cerevisiae
EDTA	complete inhibition of wild-type and mutant enzymes	Saccharomyces cerevisiae
Mg2+	inhibits the wild-type Chs2	Saccharomyces cerevisiae
Ni2+	inhibits the wild-type enzyme and mutant Chs2DELTAN222	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Saccharomyces cerevisiae	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates mutant Chs2DELTAN222	Saccharomyces cerevisiae
Mn2+	activates the wild-type Chs2 and mutant Chs2DELTAN222	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Saccharomyces cerevisiae	Chs2 synthesizes the primary septum after mitosis is completed. It is essential for proper cell separation and expected to be highly regulated. Chs2 is hyperactivated by a soluble yeast protease, which is expressed during logarithmic growth phase, when budding cells require Chs2 activity	?	-	?
additional information	Saccharomyces cerevisiae Y3437	Chs2 synthesizes the primary septum after mitosis is completed. It is essential for proper cell separation and expected to be highly regulated. Chs2 is hyperactivated by a soluble yeast protease, which is expressed during logarithmic growth phase, when budding cells require Chs2 activity	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	isozyme Chs2	-
Saccharomyces cerevisiae Y3437	-	isozyme Chs2	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation site mapping, Chs2 contains twelve phosphorylation sites, all in the N-terminal domain, phosphorylation of the N-terminal domain is important for Chs2 stability, these sites might play an important role in the cell cycle-dependent degradation of the enzyme, and thus in cell division	Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment)	Organism
partial purification of recombinant Chs2 from Pichia pastoris membranes by nickel affinity chromatography	Saccharomyces cerevisiae

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	expression, localization and degradation of Chs2 are cell cycle dependent	Saccharomyces cerevisiae	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Chs2 synthesizes the primary septum after mitosis is completed. It is essential for proper cell separation and expected to be highly regulated. Chs2 is hyperactivated by a soluble yeast protease, which is expressed during logarithmic growth phase, when budding cells require Chs2 activity	Saccharomyces cerevisiae	?	-	?
additional information	Chs2 synthesizes the primary septum after mitosis is completed. It is essential for proper cell separation and expected to be highly regulated. Chs2 is hyperactivated by a soluble yeast protease, which is expressed during logarithmic growth phase, when budding cells require Chs2 activity	Saccharomyces cerevisiae Y3437	?	-	?

Synonyms

Synonyms	Comment	Organism
chitin synthase 2	-	Saccharomyces cerevisiae
Chs2	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8.5	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)