Literature summary for 2.4.1.16 extracted from

Uchida, Y.; Shimmi, O.; Sudoh, M.; Arisawa, M.; Yamada-Okabe, H.
Characterization of chitin synthase 2 of Saccharomyces cerevisiae II: Both full size and processed enzymes are active for chitin synthesis (1996), J. Biochem., 119, 659-666.

Search for more literature for 2.4.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
since chitin synthase 2 can not expressed in bacterial cells or insect cells in an active form, Saccharomyces cerevisiae is used as the host for overexpression	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Nikkomycin	nikkomycin Z	Saccharomyces cerevisiae
nikkomycin Z	-	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	significant chitin synthase activity in the presence of Mg2+ or Mn2+, even without trypsin treatment	Saccharomyces cerevisiae
Mn2+	significant chitin synthase activity in the presence of Mg2+ or Mn2+, even without trypsin treatment	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	chitin synthetase 2	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	chitin synthase 2 activity is independent of the N-terminal 193 amino acid truncation, because partially purified full length enzyme also exhibits the activity without trypsin treatment in the presence of appropriate cations	Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n	-	Saccharomyces cerevisiae	UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0068	-	nikkomycin Z	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)