Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GlcNAc | stimulates both Chs1 and Chs2 about 3fold | Saccharomyces cerevisiae | |
papain | fairly good activator of Chs1, poor activator of Chs2 | Saccharomyces cerevisiae | |
Pronase | stimulates both Chs1 and Chs2 | Saccharomyces cerevisiae | |
proteinase B | from Saccharomyces cerevisiae, stimulates Chs1, no effect on Chs2 | Saccharomyces cerevisiae | |
proteinase K | stimulates both Chs1 and Chs2 | Saccharomyces cerevisiae | |
Staphylococcus V8 protease | best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits Chs1 | Saccharomyces cerevisiae | |
EDTA | 0.1 mM, Chs1 and Chs2 are almost completely inhibited | Saccharomyces cerevisiae | |
NaCl | 0.5 M, 86% inhibition of Chs1 and 29% inhibition of Chs2 | Saccharomyces cerevisiae | |
Polyoxin D | Chs2 shows less sensitivity to inhibition than Chs1 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
UDP-GlcNAc | Chs1 | Saccharomyces cerevisiae | |
0.9 | - |
UDP-GlcNAc | Chs1 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | most of the enzyme is associated with | Saccharomyces cerevisiae | 5886 | - |
protoplast | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | in stimulation of Chs2 Co2+ is twice as effective on an enzyme activated by trypsin | Saccharomyces cerevisiae | |
Mg2+ | best stimulator of Chs1. Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 | Saccharomyces cerevisiae | |
Mn2+ | Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | Saccharomyces cerevisiae | chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
chitin synthetase 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | pronase and proteinase K stimulates both Chs1 and Chs2. Proteinase B from Saccharomyces cerevisiae stimulates Chs1 and has no effect on Chs2, Staphylococcus V8 protease is the best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | - |
Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | chitin | ? | |
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene | Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Polyoxin D | Chs1 | Saccharomyces cerevisiae | |
0.015 | - |
Polyoxin D | Chs2 | Saccharomyces cerevisiae |