Literature summary for 2.4.1.155 extracted from

Liu, J.; Liu, H.; Zhang, W.; Wu, Q.; Liu, W.; Liu, Y.; Pan, D.; Xu, J.; Gu, J.
N-acetylglucosaminyltransferase V confers hepatoma cells with resistance to anoikis through EGFR/PAK1 activation (2013), Glycobiology, 23, 1097-1109.

Search for more literature for 2.4.1.155

Cloned(Commentary)

Cloned (Comment)	Organism
Huh7 and HepG2 cells are transiently transfected by both Mgat5 expression vector plasmid and empty vector plasmid as a control	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L188R	site-directed mutagenesis, inactive mutant	Homo sapiens
additional information	generation of a stable BEL7404 Tet-on-Mgat5 cell line in which human Mgat5 can be specifically induced by doxycycline to explore functional alterations after Mgat5 overexpression	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q09328	gene MGAT5	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Hep-G2 cell	the cells show lower enzyme activity	Homo sapiens	-
hepatocellular carcinoma cell	-	Homo sapiens	-
Huh-7 cell	the cells show lower enzyme activity	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
Mgat5	-	Homo sapiens
N-acetylglucosaminyltransferase V	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	Mgat5 overexpression increases p21-activated kinase 1 (PAK1) expression and upregulated beta-1-6-GlcNAc branched N-glycosylation. Mgat5 overexpression promotes anchorage-independent growth and inhibits anoikis in hepatoma cells. shRNA-mediated PAK1 knockdown and kinase inactivation with kinase dead mutant PAK1 K299R coexpression or allosteric inhibitor P21-activated kinase inhibitor III (IPA3) treatment reverses anoikis resistance in Mgat5-overexpressed hepatoma cells. Knockdown of Mgat5 reduces EGFR/PAK1-dependent anoikis resistance, which can be reversed by PAK1 T423E	Homo sapiens
physiological function	elevated expression and activity of N-acetylglucosaminyltransferase V in hepatocellular carcinoma is a common early event involved in tumor invasion during hepatocarcinogenesis. EGFR/PAK1 signaling dominates Mgat5-induced resistance of hepatoma cells to anoikis	Homo sapiens

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Release 2023.1 (January 2023)