Literature summary for 2.4.1.155 extracted from

Bubka, M.; Link-Lenczowski, P.; Janik, M.; Pochec, E.; Litynska, A.
Overexpression of N-acetylglucosaminyltransferases III and V in human melanoma cells. Implications for MCAM N-glycosylation (2014), Biochimie, 103, 37-49.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144	Homo sapiens

Cloned (Comment)

Organism

gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Homo sapiens	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases	?	-	?
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R	Homo sapiens	-	UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q09328	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
melanoma cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases	Homo sapiens	?	-	?
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R	-	Homo sapiens	UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R	-	?

Synonyms

Synonyms	Comment	Organism
GnT-IV	-	Homo sapiens
Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase V	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	N-acetylglucosaminyltransferase V catalyzes the synthesis of the beta1-6 bond between the sugar residue of the donor and alpha1,6-linked mannose of the core of N-glycan. Subsequently, the beta1-6 branch is formed and antennae are initiated	Homo sapiens