Literature summary for 2.4.1.155 extracted from

Palcic, M.M.; Ripka, J.; Kaur, K.J.; Shoreibah, M.; Hindsgaul, O.; Pierce, M.
Regulation of N-acetylglucosaminyltransferase V activity. Kinetic comparisons of parental, Rous sarcoma virus-transformed BHK, and L-phytohemagglutinin-resistant BHK cells using synthetic substrates and an inhibitory substrate analog (1990), J. Biol. Chem., 265, 6759-6769.

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Inhibitors

Inhibitors	Comment	Organism	Structure
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3	-	Mesocricetus auratus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	1.2	UDP-N-acetylglucosamine	-	Mesocricetus auratus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Mesocricetus auratus	16020	-
microsome	-	Mesocricetus auratus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no requirement for Mn2+	Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	Mesocricetus auratus	involved in biosynthesis of branched N-glycopeptides	UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	-	?

Organism

Organism	UniProt	Comment	Textmining
Mesocricetus auratus	-	hamster	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
BHK-21 cell	native BHK-21/c13 cells, cells transformed with Rous sarcoma virus, i.e. RS-BHK-cells, and lectin-resistant cell-lines LP 1.6 and 3.3	Mesocricetus auratus	-
kidney	-	Mesocricetus auratus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mesocricetus auratus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	synthetic acceptor substrates	Mesocricetus auratus	UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	-	?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	involved in biosynthesis of branched N-glycopeptides	Mesocricetus auratus	UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Mesocricetus auratus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.06	0.077	N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3	-	Mesocricetus auratus

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Release 2023.1 (January 2023)