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Literature summary for 2.4.1.153 extracted from

  • Larkin, A.; Chang, M.M.; Whitworth, G.E.; Imperiali, B.
    Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis (2013), Nat. Chem. Biol., 9, 367-373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli with N-terminal T7 and C-terminal His6 tags Methanococcus voltae

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Methanococcus voltae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-D-glucosamine to the membrane bound acceptor dolichyl phosphate. The recombinant enzyme produced in Escherichia coli sediments with the membrane fraction and requires detergent for solubility, suggesting it may contain hydrophobic regions that associate with the membrane Methanococcus voltae 5737
-
membrane the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate. The recombinant enzyme produced in Escherichia coli sediments with the membrane fraction and requires detergent for solubility, suggesting it may contain hydrophobic regions that associate with the membrane Methanococcus voltae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ Methanococcus voltae
Mg2+ enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ Methanococcus voltae
Mn2+ enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ Methanococcus voltae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28175
-
x * 28175, calculated from sequence Methanococcus voltae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate Methanococcus voltae the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate Methanococcus voltae PS the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Methanococcus voltae B3VA58
-
-
Methanococcus voltae PS B3VA58
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanococcus voltae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate Methanococcus voltae UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate the enzyme shows much higher activity with the shorter, native-like (C55–60) dolichyl monophosphate substrate compared with the longer (C85–105) dolichyl monophosphate, indicating a strong preference of the enzyme for the shorter dolichols found in archaea Methanococcus voltae UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate Methanococcus voltae PS UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate the enzyme shows much higher activity with the shorter, native-like (C55–60) dolichyl monophosphate substrate compared with the longer (C85–105) dolichyl monophosphate, indicating a strong preference of the enzyme for the shorter dolichols found in archaea Methanococcus voltae PS UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 28175, calculated from sequence Methanococcus voltae

Synonyms

Synonyms Comment Organism
AglK
-
Methanococcus voltae
Dol-P-GlcNAc synthase
-
Methanococcus voltae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Methanococcus voltae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanococcus voltae

General Information

General Information Comment Organism
metabolism the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate Methanococcus voltae