Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli with N-terminal T7 and C-terminal His6 tags | Methanococcus voltae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Methanococcus voltae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-D-glucosamine to the membrane bound acceptor dolichyl phosphate. The recombinant enzyme produced in Escherichia coli sediments with the membrane fraction and requires detergent for solubility, suggesting it may contain hydrophobic regions that associate with the membrane | Methanococcus voltae | 5737 | - |
membrane | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate. The recombinant enzyme produced in Escherichia coli sediments with the membrane fraction and requires detergent for solubility, suggesting it may contain hydrophobic regions that associate with the membrane | Methanococcus voltae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ | Methanococcus voltae | |
Mg2+ | enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ | Methanococcus voltae | |
Mn2+ | enzymatic activity requires divalent metal cations Ca2+, Mn2+, or Mg2+ | Methanococcus voltae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28175 | - |
x * 28175, calculated from sequence | Methanococcus voltae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | Methanococcus voltae | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | Methanococcus voltae PS | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus voltae | B3VA58 | - |
- |
Methanococcus voltae PS | B3VA58 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanococcus voltae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate | Methanococcus voltae | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | the enzyme shows much higher activity with the shorter, native-like (C5560) dolichyl monophosphate substrate compared with the longer (C85105) dolichyl monophosphate, indicating a strong preference of the enzyme for the shorter dolichols found in archaea | Methanococcus voltae | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate | Methanococcus voltae PS | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | the enzyme shows much higher activity with the shorter, native-like (C5560) dolichyl monophosphate substrate compared with the longer (C85105) dolichyl monophosphate, indicating a strong preference of the enzyme for the shorter dolichols found in archaea | Methanococcus voltae PS | UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28175, calculated from sequence | Methanococcus voltae |
Synonyms | Comment | Organism |
---|---|---|
AglK | - |
Methanococcus voltae |
Dol-P-GlcNAc synthase | - |
Methanococcus voltae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methanococcus voltae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanococcus voltae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme initiates the N-linked glycan biosynthesis in the cytoplasm by transfer of N-acetyl-alpha-D-glucosamine to the membrane bound acceptor dolichyl phosphate | Methanococcus voltae |