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Literature summary for 2.4.1.152 extracted from

  • Ahsen, O.; Voigtmann, U.; Klotz, M.; Nifantiev, N.; Schottelius, A.; Ernst, A.; Mueller-Tiemann, B.; Parczyk, K.
    A miniaturized high-throughput screening assay for fucosyltransferase VII (2008), Anal. Biochem., 372, 96-105.
    View publication on PubMed
Search for more literature for 2.4.1.152

Application

Application Comment Organism
drug development fucosyltransferase VII is a very promising drug target for treatment of inflammatory skin diseases Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of FucTVII catalytic domain in HEK-293 cells Homo sapiens

General Stability

General Stability Organism
FucTVII is very stable against freeze/thaw cycles Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-F-GDP-fucose complete inhibition Homo sapiens
GDP-fucose competitive inhibition Homo sapiens
additional information high-throughput screening for FucTVII inhibitors, overview Homo sapiens
Neu5(OHAc)alpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)6-deoxyGlcNAc-beta-methyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)6-sulfoGlcNAc-beta-methyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-3)LacN-(2-acetamido)ethyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-6)GalNAc-(2-acetamido)ethyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-biphenyl-4-carboxylic acid propyl amide
-
 Homo sapiens
Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-methyl
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 GDP-beta-L-fucose pH 7.4, 22°C Homo sapiens
0.05
-
 Neu5Acalpha(2,3)-Galbeta(1,4)-6-O-sulfo-GlcNAc beta-O-C3H6-biotin pH 7.4, 22°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ preferred divalent cation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + Neu5Acalpha-(2,3)-Galbeta-(1,4)-GlcNAcbeta-R Homo sapiens the last step in sLex epitope formation is catalyzed byalpha(1,3)-fucosyltransferase VII, i.e. FucTVII, which transfers a fucosyl moiety from GDP-fucose to the core oligosaccharide structures, overview GDP + Neu5Acalpha-(2,3)-Galbeta-(1,4)-[Fucalpha-(1,3)]-GlcNAcbeta-R i.e. antigen sLex ?
additional information Homo sapiens the natural substrate of FucTVII is a glycoprotein carrying 3'SLN-terminated polylactoseamine chains, FucTVII activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + fetuin
-
 Homo sapiens ?
-
 ?
GDP-beta-L-fucose + fetuin-biotin
-
 Homo sapiens ?
-
 ?
GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-6-O-sulfo-GlcNAc beta-O-C3H6-biotin i.e. 6-sulfo-3'SLN, best substrate Homo sapiens ?
-
 ?
GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-(1,3)-Galbeta-(1,4)-Glcbeta-O-C3H6-biotin i.e. 3'SLNL Homo sapiens ?
-
 ?
GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-O-BSA-biotin i.e. 3'SLN-BSA Homo sapiens ?
-
 ?
GDP-beta-L-fucose + Neu5Ac alpha(2,3)-Galbeta(1,4)-GlcNAc beta-O-C3H6-biotin i.e. 3'SLN Homo sapiens ?
-
 ?
GDP-beta-L-fucose + Neu5Acalpha-(2,3)-Galbeta-(1,4)-GlcNAcbeta-R the last step in sLex epitope formation is catalyzed byalpha(1,3)-fucosyltransferase VII, i.e. FucTVII, which transfers a fucosyl moiety from GDP-fucose to the core oligosaccharide structures, overview Homo sapiens GDP + Neu5Acalpha-(2,3)-Galbeta-(1,4)-[Fucalpha-(1,3)]-GlcNAcbeta-R i.e. antigen sLex ?
additional information the natural substrate of FucTVII is a glycoprotein carrying 3'SLN-terminated polylactoseamine chains, FucTVII activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha(1,3)-fucosyltransferase VII
-
 Homo sapiens
FucTVII
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 6-F-GDP-fucose pH 7.4, 22°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00064
-
 pH 7.4, 22°C Homo sapiens 6-F-GDP-fucose
0.0104
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)6-sulfoGlcNAc-beta-methyl
0.0143
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-biphenyl-4-carboxylic acid propyl amide
0.0279
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)6-deoxyGlcNAc-beta-methyl
0.0347
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-6)GalNAc-(2-acetamido)ethyl
0.035
-
 pH 7.4, 22°C Homo sapiens Neu5(OHAc)alpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl
0.0516
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-methyl
0.0838
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta(1-3)LacN-(2-acetamido)ethyl
0.227
-
 pH 7.4, 22°C Homo sapiens Neu5Acalpha(2-3)Galbeta(1-4)GlcNAc-beta-(3-amino)propyl