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Literature summary for 2.4.1.152 extracted from

  • Sarnesto, A.; Kohlin, T.; Hindsgaul, O.; Vogele, K.; Blaszczyk-Thurin, M.; Thurin, J.
    Purification of the beta-N-acetylglucosaminide alpha 1,3-fucosyltransferase from human serum (1992), J. Biol. Chem., 267, 2745-2752.
    View publication on PubMed
Search for more literature for 2.4.1.152

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 GDP-fucose
-
 Homo sapiens
0.53
-
 N-acetyl-beta-lactosamine
-
 Homo sapiens
0.76
-
 lacto-N-neotetraose
-
 Homo sapiens
0.96
-
 NeuAcalpha2-3Galbeta1-4GlcNAc
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation, hydrophobic chromatography in phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-heanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc
-
 Homo sapiens GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc
-
 ?
GDP-fucose + lacto-N-neotetraose
-
 Homo sapiens GDP + ?
-
 ?
GDP-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc
-
 Homo sapiens GDP + NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
additional information lactose and its fucose-substituted derivative are very poor acceptors for the enzyme Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Homo sapiens