Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | 80% inhibition at 0.4 mM | Homo sapiens | |
N-ethylmaleimide | not inhibitory at 5 mM, 30 min. | Homo sapiens | |
NaCl | 40% inhibition at 300 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
GDP-fucose | - |
Homo sapiens | |
0.4 | - |
Galbeta1-4GlcNAc | - |
Homo sapiens | |
1 | - |
Fucalpha1-2Galbeta1-4Glc | - |
Homo sapiens | |
8.1 | - |
Galbeta1-4Glc | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | optimal activity at 5 mM | Homo sapiens | |
Zn2+ | at 10 mM stimulates the activity more than Mn2+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using method A: column chromatography on DEAE-Sephadex, SP-Sephadex, ASTD-Sepharose and GTP-Agarose, method B: column chromatography on DEAE-Sephacel, SP-Sephadex and Synsorb-Galbeta1-4GlcNAc | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuroblastoma cell | CHP 134 | Homo sapiens | - |
serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc | - |
Homo sapiens | GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc | - |
? | |
GDP-fucose + Galbeta1-4Glc | - |
Homo sapiens | GDP + Galbeta1-4 (Fucalpha1-3)Glc | - |
? | |
GDP-fucose + Galbeta1-4GlcNAc | - |
Homo sapiens | GDP + Galbeta1-4 (Fucalpha1-3)GlcNAc | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Homo sapiens | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-45000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.25 | - |
- |
Homo sapiens |