Application | Comment | Organism |
---|---|---|
drug development | TPS is a potential insecticidal molecular target, 4-substituted 2,6-diamino-3,5-dicyano-4H-thiopyrans may serve as lead compounds for the development of insecticides with a distinct mode of action | Ctenocephalides felis |
drug development | TPS is a potential insecticidal molecular target, 4-substituted 2,6-diamino-3,5-dicyano-4H-thiopyrans may serve as lead compounds in for the development of insecticides with a distinct mode of action | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
gene cftps, DNA and amino acid sequence determination and anaysis, phylogenetic analysis, expression of largely soluble MalE-CfTPS fusion protein in Escherichia coli Rosetta (DE3) cells | Ctenocephalides felis |
gene dmtps1, phylgenetic analysis, expression of His-tagged TPS1 in Escherichia coli strain BL21 | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile | - |
Ctenocephalides felis | |
3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile | - |
Drosophila melanogaster | |
6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile | - |
Ctenocephalides felis | |
6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile | - |
Drosophila melanogaster | |
8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine | - |
Ctenocephalides felis | |
8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine | - |
Drosophila melanogaster | |
8-hydroxy-2,4-dimethyl-7H-chromen-7-one | - |
Ctenocephalides felis | |
8-hydroxy-2,4-dimethyl-7H-chromen-7-one | - |
Drosophila melanogaster | |
methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate | - |
Ctenocephalides felis | |
methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate | - |
Drosophila melanogaster | |
additional information | inhibitor screening amongst over 115000 synthetic compounds, structure-function relationship analysis of 4-substituted 2,6-diamino-3,5-dicyano-4H-thiopyrans, overview | Ctenocephalides felis | |
additional information | inhibitor screening amongst over 115000 synthetic compounds, structure-function relationship analysis of 4-substituted 2,6-diamino-3,5-dicyano-4H-thiopyrans, overview | Drosophila melanogaster | |
O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate | - |
Ctenocephalides felis | |
O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Drosophila melanogaster | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | Ctenocephalides felis | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ctenocephalides felis | J7G8S5 | gene cftps; gene cftps | - |
Drosophila melanogaster | Q9Y119 | TPS1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged TPS1 from Escherichia coli strain BL21 by nickel affinity chromatography | Drosophila melanogaster |
recombinant soluble MalE-CfTPS fusion protein from Escherichia coli Rosetta (DE3) cells partially by by amylose-agarose affinity chromatography | Ctenocephalides felis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Drosophila melanogaster | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Ctenocephalides felis | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | the enzyme is substrate-specific | Drosophila melanogaster | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | the enzyme is substrate-specific | Ctenocephalides felis | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Drosophila melanogaster |
TPS | - |
Ctenocephalides felis |
TPS1 | - |
Drosophila melanogaster |
trehalose phosphate synthase | - |
Drosophila melanogaster |
trehalose phosphate synthase | - |
Ctenocephalides felis |
trehalose-6-phosphate synthase | - |
Drosophila melanogaster |
trehalose-6-phosphate synthase | - |
Ctenocephalides felis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Drosophila melanogaster |
22 | - |
assay at room temperature | Ctenocephalides felis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Drosophila melanogaster |
7.5 | - |
assay at | Ctenocephalides felis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0005 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0008 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0012 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0012 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0013 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0014 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile | |
0.0014 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile | |
0.0017 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0019 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile | |
0.0026 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0026 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0028 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate | |
0.0029 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0031 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.00371 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | |
0.004 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0045 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0045 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0046 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile | |
0.0046 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0049 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.005 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.00505 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | |
0.0054 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0055 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate | |
0.0059 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0061 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0061 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0062 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine | |
0.0066 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine | |
0.0086 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 8-hydroxy-2,4-dimethyl-7H-chromen-7-one | |
0.0094 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate | |
0.011 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0129 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0147 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate | |
0.0149 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0173 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.023 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0287 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0314 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 8-hydroxy-2,4-dimethyl-7H-chromen-7-one | |
0.0419 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0631 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0665 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0758 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.0918 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile | |
0.1 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Ctenocephalides felis | 6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile | |
789 | - |
2 mM phosphoenol pyruvate, 2% (w/v) NaCl, 0.5% (w/v) KCl, 10 mM MgCl2, 1 mM dithiothreitol, 1.5 U/ml pyruvate kinase, pH 7.5 22 °C | Drosophila melanogaster | 2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile |
General Information | Comment | Organism |
---|---|---|
physiological function | trehalose phosphate synthase is the crucial enzyme for the biosynthesis of trehalose, the main haemolymph sugar of insects | Drosophila melanogaster |
physiological function | trehalose phosphate synthase is the crucial enzyme for the biosynthesis of trehalose, the main haemolymph sugar of insects | Ctenocephalides felis |