Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.15 extracted from

  • Lippert, K.; Galinski, E.A.; Trueper, H.G.
    Biosynthesis and function of trehalose in Ectothiorhodospira halochloris (1993), Antonie van Leeuwenhoek, 63, 85-91.
    View publication on PubMed
Search for more literature for 2.4.1.15

General Stability

General Stability Organism
stabilization during purification, 10fold by addition of 20 mM MgCl2, 50 mM NaCl, 2 M glycine betaine and 5 mM mercaptoethanol Halorhodospira halochloris

Inhibitors

Inhibitors Comment Organism Structure
trehalose non-competitive Halorhodospira halochloris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 UDPglucose
-
 Halorhodospira halochloris
2
-
 glucose 6-phosphate
-
 Halorhodospira halochloris

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Halorhodospira halochloris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KHCO3 100-300 mM, activation Halorhodospira halochloris
Mg2+ optimal concentration: 3-6 mM MgCl2 Halorhodospira halochloris
NaHCO3 100-300 mM, activation Halorhodospira halochloris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Halorhodospira halochloris

Organism

Organism UniProt Comment Textmining
Halorhodospira halochloris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Halorhodospira halochloris

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate ordered bi-bi mechanism Halorhodospira halochloris
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.127
-
 UDPglucose Halorhodospira halochloris

Storage Stability

Storage Stability Organism
-18°C, 2-3 d, no loss of activity Halorhodospira halochloris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + glucose 6-phosphate specific for UDPglucose, no reaction with ADPglucose or GDPglucose Halorhodospira halochloris trehalose 6-phosphate + UDP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Halorhodospira halochloris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 potassium phosphate buffer Halorhodospira halochloris
7
-
 Tris/HCl buffer Halorhodospira halochloris