Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPSO | activates solubilized enzyme | Homo sapiens | |
deoxycholate | activates solubilized enzyme | Homo sapiens | |
G-3634-A | activates solubilized enzyme | Homo sapiens | |
additional information | CDPcholine stimulates by protecting UDP-GlcNAc from hydrolysis by endogenous enzymes | Homo sapiens | |
Triton CF-54 | activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3% | Homo sapiens | |
Triton X-100 | activates solubilized enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
UDP-N-acetyl-D-glucosamine | lactosylceramide as acceptor | Homo sapiens | |
0.19 | - |
lactoneotetraosylceramide | - |
Homo sapiens | |
0.19 | - |
lactonorhexaosylceramide | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | strict requirement | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme is undetectable in normal colonic epithelial cells, but is greatly enhanced both during fetal development and in colonic adenocarcinomas | Homo sapiens | - |
SW-403 cell | colonic adenocarcinoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | transfer of GlcNAc occurs mainly to type 2 chain nonfucosylated structures, elongation of type 1 chain structure Lc4 is also detectable, no transfer to any fucosylated derivative of either type 1 or 2 chains, transfer of GlcNAc to a terminal Gal residue on a lacto-series core chain | Homo sapiens | ? | - |
? | |
additional information | biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia | Homo sapiens | ? | - |
? | |
UDP-N-acetylglucosamine + lactoneotetraosylceramide | lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length | Homo sapiens | ? | - |
? | |
UDP-N-acetylglucosamine + lactonorhexaosylceramide | lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length | Homo sapiens | ? | - |
? | |
UDP-N-acetylglucosamine + lactosylceramide | lactosylceramide from human erythrocytes | Homo sapiens | ? | - |
? | |
UDP-N-acetylglucosamine + lactotetraosylceramide | lactotetraosylceramide from human meconium, poor substrate | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.5 | broad | Homo sapiens |
7 | 7.2 | highest activity, with Hepes buffer | Homo sapiens |