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Literature summary for 2.4.1.147 extracted from
- High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis (1989), Methods Enzymol., 179, 351-397.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v | Rattus norvegicus |  |
| Triton X-100 | stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v | Sus scrofa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
N-acetyl-D-galactosaminyl-alpha-benzyl | - |
Sus scrofa | |
| 3.2 | - |
N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
Ovis aries | |
| 5 | - |
N-acetyl-D-galactosaminyl-alpha-phenyl | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R | Rattus norvegicus | R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| no activity in Canis familiaris | - |
submaxillary glands | - |
| Ovis aries | - |
- |
- |
| Platyrrhini | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colonic mucosa | - |
Homo sapiens | - |
| colonic mucosa | - |
Rattus norvegicus | - |
| colonic mucosa | - |
Sus scrofa | - |
| colonic mucosa | - |
Platyrrhini | - |
| additional information | - |
Homo sapiens | - |
| additional information | absent from submaxillary glands | Rattus norvegicus | - |
| additional information | absent from submaxillary glands | Sus scrofa | - |
| small intestine | low activity | Rattus norvegicus | - |
| stomach | - |
Rattus norvegicus | - |
| stomach | - |
Sus scrofa | - |
| stomach | - |
Ovis aries | - |
| stomach | - |
Platyrrhini | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
Ovis aries | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R | R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | R: polypeptide derived from mucin | Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | R: polypeptide derived from mucin | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | R: polypeptide derived from mucin | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | no acceptor substrate: native ovine submaxillary mucin, containing sialyl-2,6-N-acetyl-D-galactosaminyl-groups | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Sus scrofa |
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