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Literature summary for 2.4.1.144 extracted from

  • Bubka, M.; Link-Lenczowski, P.; Janik, M.; Pochec, E.; Litynska, A.
    Overexpression of N-acetylglucosaminyltransferases III and V in human melanoma cells. Implications for MCAM N-glycosylation (2014), Biochimie, 103, 37-49.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene MGAT3, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-III, the cells show increased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT5 gene, EC 2.4.1.155 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Homo sapiens 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases ?
-
?
UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R Homo sapiens
-
UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q09327
-
-

Source Tissue

Source Tissue Comment Organism Textmining
melanoma cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases Homo sapiens ?
-
?
UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R
-
Homo sapiens UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R
-
?

Synonyms

Synonyms Comment Organism
GnT-III
-
Homo sapiens
Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase III
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function N-acetylglucosaminyltransferase III is involved in formation of beta1,4 linkage between GlcNAc and beta-mannose residue within the core of oligosaccharide. The resulting bisected N-glycan acquires a characteristic conformation that renders inaccessibility to many other enzymes participating in biosynthetic pathways of hybrid and complex multiantennary N-glycans as well as to lectins Homo sapiens