Cloned (Comment) | Organism |
---|---|
gene MGAT3, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-III, the cells show increased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT5 gene, EC 2.4.1.155 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R | Homo sapiens | - |
UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q09327 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
melanoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R | - |
Homo sapiens | UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GnT-III | - |
Homo sapiens |
Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase III | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | N-acetylglucosaminyltransferase III is involved in formation of beta1,4 linkage between GlcNAc and beta-mannose residue within the core of oligosaccharide. The resulting bisected N-glycan acquires a characteristic conformation that renders inaccessibility to many other enzymes participating in biosynthetic pathways of hybrid and complex multiantennary N-glycans as well as to lectins | Homo sapiens |