Cloned (Comment) | Organism |
---|---|
amplified for cloning into pENTR-D-Topo vector, cloned gene inserted into the virus expression vector, pBABE-puro. GnT-III and GnT-V constructs transfected into Phoenix-Ampho cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GnT-III selectively modifies N-glycosylation site-4 on the integrin alpha5 subunit (S-3,4,5), which down-regulates its biological functions | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-N-acetylglucosaminyltransferase III | - |
Homo sapiens |
GnT-III | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | introduction of GnT-III suppresses additional processing and branching formation of N-glycans catalyzed by other endogenous glycosyltransferases, such as GnT-V and GnT-IV. Cell adhesion on fibronectin is down-regulated in GnT-III transfectants compared with mock and GnT-V transfectants. Overexpression of GnT-III significantly inhibits cell migration on fibronectin. GnT-III significantly down-regulates cell spreading on fibronectin in wild-type transfectants, whereas the deletion of site-4 abolishes the suppression of cell spread induced by GnT-III in D-4 transfectants | Homo sapiens |