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Literature summary for 2.4.1.142 extracted from
- Identification and characterization of transcriptional control region of the human beta 1,4-mannosyltransferase gene (2017), Cytotechnology, 69, 417-434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HMT-1, DNA and amino acid sequence determination and analysis, cloning from HeLa cells, subcloning in Escherichia coli strain JM109. Detection of potential cis-acting motifs in a 1 kb region upstream from initiation codon of the HMT-1 and analysis of transcriptional regulation of gene HMT-1, determination of initiation site for the HMT-1 transcription, overview. Semi-quantitative RT-PCR enzyme expression analysis. Expression profile of the HMT-1 among various tissues, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| rough endoplasmic reticulum membrane | luminal side | Homo sapiens | 30867 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose + chitobiosyldiphosphodolichol | Homo sapiens | - |
GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BT22 | gene HMT-1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| additional information | tissue specific enzyme expression analysis: the HMT-1 expression is generally upregulated in liver, kidney, spleen, pancreas, testis and ovary, possibly reflecting high demand of N-glycans for proteins produced in these tissues. Inversely, its expression is downregulated in skeletal muscle, thymus, prostate and small intestine for unknown reason | Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thymus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-mannose + chitobiosyldiphosphodolichol | - |
Homo sapiens | GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta1,4-MT | - |
Homo sapiens |
| chitobiosyldiphosphodolichol beta-mannosyltransferase | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | for proper accomplishment of protein N-glycosylation, early assembly of dolichol-linked oligosaccharides on cytoplasmic side of the rough endoplasmic reticulum membrane must progress at a rate greater than subsequent flipping of dolichol-linked oligosaccharides into luminal side. To assure this, all activities of five glycosyltransferases involved in early DLO assembly are required in addition to their substrates. If any of them is diminished, eukaryotic cell would not be able to survive | Homo sapiens |
| physiological function | the enzyme is involved in the early assembly of dolichol-linked oligosaccharides. Early dolichol-linked oligosaccharide assembly from dolichyl phosphate to Man5GlcNAc2-PP-Dol is a fundamental and essential event for viability of eukaryotic cells, especially multicellular organisms. Analysis of transcriptional regulation, overview. The 200 bp region upstream from HMT-1 open reading frame is crucial for both positive and negative regulation of the HMT-1 transcription | Homo sapiens |
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