Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.142 extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Purification and properties of beta-mannosyltransferase that synthesizes Man-beta-GlcNAc-GlcNAc-pyrophosphoryl-dolichol (1986), Arch. Biochem. Biophys., 250, 38-47.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NP-40 optimum activity at 0.1% Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
Sus scrofa
GDP-glucose
-
Sus scrofa
GMP
-
Sus scrofa
GTP
-
Sus scrofa
p-chloromercuribenzenesulfonic acid
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
GDP-mannose
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ less stimulatory than Mg2+ Sus scrofa
Mg2+ absolute requirement for activity, optimum activity at 5mM Sus scrofa
Mn2+ less stimulatory than Mg2+ Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-alpha-D-mannose + chitobiosyldiphosphodolichol Sus scrofa
-
GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, epoxy-activated-Sepharose Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-18ºC, 0.5 mM DTT, 20% glycerol, several weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannose + chitobiosyldiphosphodolichol
-
Sus scrofa GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa