Literature summary for 2.4.1.14 extracted from

Seger, M.; Gebril, S.; Tabilona, J.; Peel, A.; Sengupta-Gopalan, C.
Impact of concurrent overexpression of cytosolic glutamine synthetase (GS1) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco (2015), Planta, 241, 69-81.

Search for more literature for 2.4.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
gene SPS1, recombinant expression driven by the CaMV35S promoter in leaves of Nicotiana tabacum via Agrobacterium tumefaciens strain GV3101 transfection method, coexpression with soybean glutamine synthetase	Zea mays

Protein Variants

Protein Variants	Comment	Organism
additional information	production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + D-fructose 6-phosphate	Zea mays	-	UDP + sucrose 6F-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	P31927	gene SPS1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme partially from Nicotiana tabacum leaves	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + D-fructose 6-phosphate	-	Zea mays	UDP + sucrose 6F-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPS	-	Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	purified enzyme, 10 min, inactivation	Zea mays

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Zea mays

General Information

General Information	Comment	Organism
metabolism	the enzyme plays a key role in carbon metabolism by catalyzing the synthesis of sucrose	Zea mays

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Release 2023.1 (January 2023)