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Literature summary for 2.4.1.14 extracted from

  • Salvucci, M.E.; Drake, R.R.; Haley, B.E.
    Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves (1990), Arch. Biochem. Biophys., 281, 212-218.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-azido-UDP-glucose nearly complete inhibition at 1 mM Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
UDP-glucose
-
Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
SDS-PAGE Spinacia oleracea
253000
-
sucrose density gradient centrifugation Spinacia oleracea
413000
-
gel filtration of crude leaf extracts Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme becomes extremely unstable during the course of purification Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57
-
purified enzyme Spinacia oleracea

Storage Stability

Storage Stability Organism
-80°C, purified enzyme stable for at least 4 months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Spinacia oleracea UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 120000, sucrose density gradient centrifugation, SDS-PAGE Spinacia oleracea