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Literature summary for 2.4.1.135 extracted from

  • Tone, Y.; Pedersen, L.C.; Yamamoto, T.; Izumikawa, T.; Kitagawa, H.; Nishihara, J.; Tamura, J.; Negishi, M.; Sugahara, K.
    2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis (2008), J. Biol. Chem., 283, 16801-16807.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-1 cell, a soluble form of GlcAT-I generated by replacing the first 43 amino acids of GlcAT-I with a cleavable insulin signal sequence and a protein A IgG-binding domain Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion hanging drop method, crystal structure of the enzyme in the presence of UDP and Galbeta(1-3)Gal(6-O-sufate)beta(1-4)Xyl(2-O-phosphate)beta1-O-Ser Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 Galbeta(1-3)Galbeta(1-4)Xyl(2-O-phosphate)beta1-O-Ser pH 6.5, 2 mM MnCl2, 37°C Homo sapiens
0.035
-
 Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xyl(2-O-phosphate)beta1-O-Ser pH 6.5, 2 mM MnCl2, 37°C Homo sapiens
0.046
-
 Galbeta(1-3)Galbeta(1-4)Xylbeta1-O-Ser pH 6.5, 2 mM MnCl2, 37°C Homo sapiens
0.049
-
 Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xylbeta1-O-Ser pH 6.5, 2 mM MnCl2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 truncated, expression in COS-1 cell, a soluble form of GlcAT-I generated by replacing the first 43 amino acids of GlcAT-I with a cleavable insulin signal sequence and a protein A IgG-binding domain
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xyl(2-O-phosphate)beta1-O-Ser + UDP-GlcUA
-
 Homo sapiens GlcUAbeta(1-3)Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xyl(2-O-phosphate)beta1-O-Ser + UDP
-
 ?
Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xylbeta1-O-Ser + UDP-GlcUA
-
 Homo sapiens GlcUAbeta(1-3)Galbeta(1-3)Gal(6-O-sulfate)beta(1-4)Xylbeta1-O-Ser + UDP
-
 ?
Galbeta(1-3)Galbeta(1-4)Xyl(2-O-phosphate)beta1-O-Ser + UDP-GlcUA
-
 Homo sapiens GlcUAbeta(1-3)Galbeta(1-3)Galbeta(1-4)Xyl(2-O-phosphate)beta1-O-Ser + UDP
-
 ?
Galbeta(1-3)Galbeta(1-4)Xylbeta1-O-Ser + UDP-GlcUA
-
 Homo sapiens GlcUAbeta(1-3)Galbeta(1-3)Galbeta(1-4)Xylbeta1-O-Ser + UDP
-
 ?
additional information no substrate Gal(6-O-sufate)beta(1-3)Galbeta(1-4)Xyl(2-O-phosphate)beta1-O-Ser Homo sapiens ?
-
 ?
additional information no substrate Gal(6-O-sufate)beta(1-3)Galbeta(1-4)Xylbeta1-O-Ser Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GlcAT-I
-
 Homo sapiens
glucuronyltransferase-I
-
 Homo sapiens