Literature summary for 2.4.1.135 extracted from

Fondeur-Gelinotte, M.; Lattard, V.; Oriol, R.; Mollicone, R.; Jacquinet, J.C.; Mulliert, G.; Gulberti, S.; Netter, P.; Magdalou, J.; Ouzzine, M.; Fournel-Gigleux, S.
Phylogenetic and mutational analyses reveal key residues for UDP-glucuronic acid binding and activity of beta1,3-glucuronosyltransferase I (GlcAT-I) (2006), Protein Sci., 15, 1667-1678.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D113A	inactive mutant enzyme. Mutant enzyme is produced in a slightly lower amount compared with the wild-type enzyme	Homo sapiens
D113E	KM-value for UDP-glucuronate is 3.2fold higher than wild-type value	Homo sapiens
D113N	KM-value for UDP-glucuronate is 12.7fold higher than wild-type value	Homo sapiens
R156A	inactive mutant enzyme	Homo sapiens
R156K	inactive mutant enzyme	Homo sapiens
R161A	inactive mutant enzyme	Homo sapiens
R161K	KM-value for UDP-glucuronate is 21.2fold higher than wild-type value	Homo sapiens
R310A	KM-value for UDP-glucuronate is 3.5fold higher than wild-type value	Homo sapiens
R310K	KM-value for UDP-glucuronate is identical to wild-type value	Homo sapiens
R310Q	KM-value for UDP-glucuronate is 1.5fold lower than wild-type value	Homo sapiens
Y84A	inactive mutant enzyme, slightly less expressed than wild-type enzyme	Homo sapiens
Y84F	mutant enzyme shows 60% of wild-type activity. Mutant enzyme is produced at higher level than the wild-type protein. KM-value for UDP-glucuronate is 3fold higher than wild-type value	Homo sapiens
Y84H	mutant retains 21% of the activity with a KM value double that of the wild type. KM-value for UDP-glucuronate is 2.3fold higher than wild-type value	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme R310Q	Homo sapiens
0.06	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme R310K	Homo sapiens
0.06	-	UDP-glucuronate	37°C, pH 6.5, wild-type enzyme	Homo sapiens
0.14	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme Y84H	Homo sapiens
0.18	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme Y84F	Homo sapiens
0.19	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme D113E	Homo sapiens
0.21	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme R310A	Homo sapiens
0.76	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme D113N	Homo sapiens
1.27	-	UDP-glucuronate	37°C, pH 6.5, mutant enzyme R161K	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein	Homo sapiens	plays a key role in glycosaminoglycan biosynthesis	UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein	plays a key role in glycosaminoglycan biosynthesis	Homo sapiens	UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein	-	?

Synonyms

Synonyms	Comment	Organism
beta1,3-glucuronosyltransferase I	-	Homo sapiens
GlcAT-I	-	Homo sapiens

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Release 2023.1 (January 2023)