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Literature summary for 2.4.1.133 extracted from
- Biosynthesis of chondroitin sulfate. Role of phospholipids in the activity of UDP-D-galactose: D-xylose galactosyltransferase (1976), J. Biol. Chem., 251, 285-291.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against detergent-free buffer, +/- 0.25 M KCl inactivates, dialysis against 1% Nonidet P-40 and 0.25 M KCl restores activity, even after a period of 18 h in the absence of detergent | Gallus gallus |
| Nonidet P-40 stabilizes solubilized enzyme | Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gallus gallus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | requirement, 15 mM | Gallus gallus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPgalactose + O-beta-D-xylosylprotein | Gallus gallus | involved in chondroitin sulfate biosynthesis | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized and partially purified, affinity chromatography | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cartilage | epiphyses from embryonic femurs and tibiae | Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactose + D-xylose | - |
Gallus gallus | UDP + 4-O-beta-D-galactosyl-beta-D-xylose | - |
? | |
| UDPgalactose + O-beta-D-xylosylprotein | involved in chondroitin sulfate biosynthesis | Gallus gallus | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? | |
| UDPgalactose + xylosylserine | - |
Gallus gallus | UDP + beta-D-galactosyl-1,4-beta-D-xylosylserine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
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Release 2023.1 (January 2023)
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