BRENDA - Enzyme Database
show all sequences of 2.4.1.132

The biosynthesis of oligosaccharide-lipids. Activation of mannosyltransferase II by specific phospholipids

Jensen, J.W.; Schutzbach, J.S.; J. Biol. Chem. 257, 9025-9029 (1982)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cardiolipin
activation, 35% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
phosphatidylethanolamine
activation; and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases; enzyme-phospholipid-complex formation; from various eukaryotic and prokaryotic sources; in the absence of detergent; no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine; phosphoethanolamine containing unsaturated acylchains
Oryctolagus cuniculus
phosphatidylserine
activation, 16-43% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00096
-
tetrasaccharide-diphosphoryl-lipid
-
Oryctolagus cuniculus
0.00142
-
GDPmannose
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Storage Stability
Storage Stability
Organism
-20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months
Oryctolagus cuniculus
0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h
Oryctolagus cuniculus
glycerol, 10% v/v, stabilizes during storage
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
phosphatidylethanolamine increases heat-stability
Oryctolagus cuniculus
37
-
5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity
Oryctolagus cuniculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cardiolipin
activation, 35% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
phosphatidylethanolamine
activation; and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases; enzyme-phospholipid-complex formation; from various eukaryotic and prokaryotic sources; in the absence of detergent; no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine; phosphoethanolamine containing unsaturated acylchains
Oryctolagus cuniculus
phosphatidylserine
activation, 16-43% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00096
-
tetrasaccharide-diphosphoryl-lipid
-
Oryctolagus cuniculus
0.00142
-
GDPmannose
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months
Oryctolagus cuniculus
0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h
Oryctolagus cuniculus
glycerol, 10% v/v, stabilizes during storage
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
phosphatidylethanolamine increases heat-stability
Oryctolagus cuniculus
37
-
5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity
Oryctolagus cuniculus
Other publictions for EC 2.4.1.132
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736467
Imamura
Human immunodeficiency virus t ...
Mus musculus, Mus musculus C57/BL6J
J. Biol. Chem.
289
9865-9879
2014
-
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1
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1
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1
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7
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7
-
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-
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-
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-
1
3
3
1
-
-
735876
Cossins
Congenital myasthenic syndrome ...
Homo sapiens, Mus musculus
Brain
136
944-956
2013
-
-
2
-
3
-
-
-
2
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4
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1
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1
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5
5
-
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-
709008
Kämpf
Biochemical characterization a ...
Saccharomyces cerevisiae
J. Biol. Chem.
284
11900-11912
2009
-
-
-
-
20
-
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-
1
-
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1
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1
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1
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2
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1
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1
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20
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1
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1
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2
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1
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1
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707472
O'Reilly
In vitro evidence for the dual ...
Saccharomyces cerevisiae
Biochemistry
45
9593-9603
2006
-
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1
-
3
-
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1
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1
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1
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1
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3
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1
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1
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1
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3
-
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1
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1
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1
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3
-
1
-
-
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1
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-
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658848
Gao
Physical interactions between ...
Saccharomyces cerevisiae
Glycobiology
14
559-570
2004
-
-
-
-
-
-
-
-
1
-
-
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1
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1
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636759
Thiel
A new type of congenital disor ...
Homo sapiens, Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Biol. Chem.
278
22498-22505
2003
-
1
2
-
3
-
-
-
-
-
-
3
-
14
-
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1
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4
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1
3
-
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3
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3
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1
-
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4
-
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-
-
-
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1
1
-
-
-
636760
Lellouch
Phytanyl-pyrophosphate-linked ...
Komagataeibacter xylinus
Biochem. Biophys. Res. Commun.
272
290-292
2000
-
-
-
-
-
-
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1
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1
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636761
Doering
-
alpha-1,3-Mannosyltransferase ...
Cryptococcus neoformans
PCT Int. Appl.
2000
38pp
2000
-
1
-
-
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-
1
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1
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1
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1
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636757
Romero
Mnt2p and Mnt3p of Saccharomyc ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Glycobiology
9
1045-1051
1999
-
-
1
-
-
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-
1
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2
-
10
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6
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1
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6
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636763
Doering
A unique alpha-1,3-mannosyltra ...
Cryptococcus neoformans
J. Bacteriol.
181
5482-5488
1999
-
1
-
-
-
-
3
-
1
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1
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1
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1
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1
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636764
Reynolds
The high osmolarity glycerol r ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Cell Biol.
143
935-946
1998
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1
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1
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10
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636765
Wiggins
Activity of the yeast MNN1 alp ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Proc. Natl. Acad. Sci. USA
95
7945-7940
1998
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1
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10
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636766
Shpakov
Yeast dolichol-coupled mannosy ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Zh. Evol. Biokhim. Fiziol.
32
3-18
1996
-
-
1
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-
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10
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636755
Graham
Sorting of yeast alpha 1,3 man ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Mol. Biol. Cell
6
809-824
1995
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1
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1
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2
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9
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1
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636756
Graham
Clathrin-dependent localizatio ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Cell. Biol.
127
667-678
1994
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636762
Yip
Cloning and analysis of the Sa ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Proc. Natl. Acad. Sci. USA
91
2723-2727
1994
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636753
Jensen
-
Activation of mannosyltransfer ...
Oryctolagus cuniculus
Biochemistry
23
1115-1119
1984
1
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636768
Herscovics
Solubilization of an alpha-(1- ...
Bos taurus
FEBS Lett.
156
298-302
1983
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636754
Jensen
The biosynthesis of oligosacch ...
Oryctolagus cuniculus
J. Biol. Chem.
257
9025-9029
1982
3
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2
1
1
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3
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1
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636752
Jensen
The biosynthesis of oligosacch ...
Oryctolagus cuniculus
J. Biol. Chem.
256
12899-12904
1981
3
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1
15
2
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4
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15
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1
4
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1
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1
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