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Literature summary for 2.4.1.131 extracted from

  • Prakash, C.; Katial, A.; Kang, M.S.; Vijay, I.K.
    Solubilization of mannosyltransferase activities for the biosynthesis of mammary glycoproteins. Elongation of tetrasaccharide-lipid to heptasaccharide-lipid by a solubilized enzyme preparation (1984), Eur. J. Biochem., 139, 87-93.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight stimulation Bos taurus
Endogen lipids soluble in CHCl3/CH3OH, 2:1, activation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak Bos taurus
Cu2+ strong Bos taurus
Mn2+
-
Bos taurus
additional information not inhibited by amphomycin; not inhibited by EDTA Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Bos taurus 16020
-
microsome
-
Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement of divalent cations Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDPmannose + oligosaccharide-lipid acceptor Bos taurus involved in biosynthesis of lipid-linked oligosaccharides up to (Man)5-(GlcNAc)2, involved in the production of two 1,2-linked mannosyl residues in mammalian lipid-linked tetradecasaccharide of the structure (Glc)3(Man)9(GlcNAc)2, the major precursor for eukaryotic en-bloc-glycosylation of asparagin-linked glycoproteins GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
cow
-

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland lactating mammary tissue Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, solubilized enzyme, 2 weeks: about 40% loss of activity, 7 days: about 20% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + lipid-linked Manalpha(1-3)Manbeta-GlcNAcbeta-GlcNAc reaction via penta- and hexasaccharide intermediates to a lipid-linked heptasaccharide, presumably multiple mannosyltransferases involved Bos taurus GDP + lipid-linked Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta-GlcNAcbeta-GlcNAc
-
?
GDP-mannose + oligosaccharide-lipid acceptor acceptors can be mannose or Man-(GlcNAc)2 to (Man)5-(GlcNAc)2, lipid-linked tetrasaccharide Manalpha(1-3)Manbeta-GlcNAcbeta-GlcNAc, presumably multiple mannosyltransferases involved Bos taurus GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
?
GDP-mannose + oligosaccharide-lipid acceptor acceptor isolated from baby hamster kidney cells, i.e. BHK cells Bos taurus GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
?
GDPmannose + oligosaccharide-lipid acceptor involved in biosynthesis of lipid-linked oligosaccharides up to (Man)5-(GlcNAc)2, involved in the production of two 1,2-linked mannosyl residues in mammalian lipid-linked tetradecasaccharide of the structure (Glc)3(Man)9(GlcNAc)2, the major precursor for eukaryotic en-bloc-glycosylation of asparagin-linked glycoproteins Bos taurus GDP + alpha-mannosyl-1,2-oligosaccharide-lipid
-
?
additional information no sugar donor: mannosylphosphorylretinol Bos taurus additional information
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.6 broad Bos taurus