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Literature summary for 2.4.1.13 extracted from

  • Verma, E.; Sharma, B.; Singal, H.; Munjal, R.
    Purification of sucrose synthase from thermotolerant wheat grains and its characterization (2018), J. Environ. Biol., 39, 459-466 .
No PubMed abstract available
Search for more literature for 2.4.1.13

Activating Compound

Activating Compound Comment Organism Structure
NO3 20.8% increase of activity at 2 mM Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate
-
 Triticum aestivum
Mn2+ 38.5% inhibition at 2 mM Triticum aestivum
NADP+
-
 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18
-
 UDP at pH 6.5 and 37 °C Triticum aestivum
14.28
-
 sucrose at pH 6.5 and 37 °C Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
269000
-
 gel filtration Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP + sucrose the enzyme is highly specific for UDP as substrate Triticum aestivum UDP-alpha-D-glucose + D-fructose
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 63000, SDS-PAGE Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Triticum aestivum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 50 the enzyme is thermostable up to 50°C at pH 6.5 Triticum aestivum