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Literature summary for 2.4.1.13 extracted from

  • Zhang, L.; Gao, Y.; Liu, X.; Guo, F.; Ma, C.; Liang, J.; Feng, X.; Li, C.
    Mining of sucrose synthases from Glycyrrhiza uralensis and their application in the construction of an efficient UDP-recycling system (2019), J. Agric. Food Chem., 67, 11694-11702 .
    View publication on PubMed
Search for more literature for 2.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Glycyrrhiza uralensis

Protein Variants

Protein Variants Comment Organism
SUS1-DELTA9 the kcat/Km of the mutant toward sucrose isdecreased by 28.4% compared to that of wild type isoform SUS1, while the kcat/Km of the mutant toward UDP is increased by 1.6% compared to that of SUS1 Glycyrrhiza uralensis
SUS2-DELTA9 the kcat/Km of the mutant toward UDP is increased by 3.8fold compared to that of wild type isoform SUS2, while the kcat/Km of the mutant toward sucrose is decreased by 35.7% compared to that of SUS2 Glycyrrhiza uralensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
0.59
-
 UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
0.74
-
 UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
0.89
-
 UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
5.41
-
 sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
9.61
-
 sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
24.47
-
 sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
43.8
-
 sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NDP-alpha-D-glucose + D-fructose Glycyrrhiza uralensis
-
 NDP + sucrose
-
 r

Organism

Organism UniProt Comment Textmining
Glycyrrhiza uralensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Glycyrrhiza uralensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NDP-alpha-D-glucose + D-fructose
-
 Glycyrrhiza uralensis NDP + sucrose
-
 r
UDP + sucrose
-
 Glycyrrhiza uralensis UDP-alpha-D-glucose + D-fructose
-
 r

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Glycyrrhiza uralensis

Synonyms

Synonyms Comment Organism
SUS1 isoform Glycyrrhiza uralensis
SUS2 isoform Glycyrrhiza uralensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Glycyrrhiza uralensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 isoform SUS1 shows about 40% activity at 30°C, about 68% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 85% activity at 55°C, and about 35% activity at 60°C. Isoform SUS2 shows about 50% activity at 30°C, about 60% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 47% activity at 55°C, and about 30% activity at 60°C Glycyrrhiza uralensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 thermostability of wild type isoforms SUS1 and SUS2 is poor at 50°C. Activity loss occurs rapidly with increasing incubation time, and the half-lives are 50 and 25 min for SUS1 and SUS2, respectively. Mutant enzyme SUS1-DELTA9 shows a half-life increase of 2fold. The residual activity is 50% after 100 min, while the wild type activity remains less than 20% of the original activity. The half-life of the variant SUS2-DELTA9 also increases 2fold Glycyrrhiza uralensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50
-
 UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
51.2
-
 sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
62
-
 UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
74.8
-
 UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
76.8
-
 sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
87.1
-
 sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
99.6
-
 sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
121.7
-
 UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Glycyrrhiza uralensis

pH Range

pH Minimum pH Maximum Comment Organism
5 8 isoform SUS1 shows about 60% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.5, about 40% activity at pH 7.5, and about 33% activity at pH 8.0. Isoform SUS2 shows about 50% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.5, about 90% activity at pH 7.5, and about 70% activity at pH 8.0 Glycyrrhiza uralensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.29
-
 sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
3.56
-
 sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
8.01
-
 sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
11.18
-
 sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
84.3
-
 UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
84.42
-
 UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
85.75
-
 UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
407.83
-
 UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis