Literature summary for 2.4.1.13 extracted from

Gutmann, A.; Lepak, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Glycosyltransferase cascades for natural product glycosylation Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP (2017), Biotechnol. J., 12, 1600557 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Gold (DE3) cells	Glycine max
expressed in Escherichia coli BL21-Gold (DE3) cells	Acidithiobacillus caldus

Protein Variants

Protein Variants	Comment	Organism
L637M/T640V	the double mutant shows a 60fold reduced KM value for UDP and substantially improved UDP-glucose recycling. The mutant is roughly 8times more active than the wild type enzyme	Acidithiobacillus caldus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	13 mM used in assay conditions	Glycine max
Mg2+	13 mM used in assay conditions	Acidithiobacillus caldus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NDP-alpha-D-glucose + D-fructose	Glycine max	-	NDP + sucrose	-	r
NDP-alpha-D-glucose + D-fructose	Acidithiobacillus caldus	-	NDP + sucrose	-	r
NDP-alpha-D-glucose + D-fructose	Acidithiobacillus caldus ATCC 51756	-	NDP + sucrose	-	r

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus caldus	A0A059ZV61	-	-
Acidithiobacillus caldus ATCC 51756	A0A059ZV61	-	-
Glycine max	P13708	-	-

Purification (Commentary)

Purification (Comment)	Organism
His tag affinity chromatography	Acidithiobacillus caldus
Strep tag affinity chromatography	Glycine max

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NDP-alpha-D-glucose + D-fructose	-	Glycine max	NDP + sucrose	-	r
NDP-alpha-D-glucose + D-fructose	-	Acidithiobacillus caldus	NDP + sucrose	-	r
NDP-alpha-D-glucose + D-fructose	-	Acidithiobacillus caldus ATCC 51756	NDP + sucrose	-	r
UDP + sucrose	-	Glycine max	UDP-alpha-D-glucose + D-fructose	-	r
UDP + sucrose	-	Acidithiobacillus caldus	UDP-alpha-D-glucose + D-fructose	-	r
UDP + sucrose	-	Acidithiobacillus caldus ATCC 51756	UDP-alpha-D-glucose + D-fructose	-	r

Synonyms

Synonyms	Comment	Organism
SuSy	-	Glycine max
SuSy	-	Acidithiobacillus caldus

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Release 2023.1 (January 2023)