Literature summary for 2.4.1.13 extracted from

Wang, H.; Sui, X.; Guo, J.; Wang, Z.; Cheng, J.; Ma, S.; Li, X.; Zhang, Z.
Antisense suppression of cucumber (Cucumis sativus L.) sucrose synthase 3 (CsSUS3) reduces hypoxic stress tolerance (2014), Plant Cell Environ., 37, 795-810.

Search for more literature for 2.4.1.13

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	when suffering hypoxia stress from flooding, CsSUS3 expression and SUS activity in roots increase, especially in the lateral roots. The soluble SUS activity increases, but the membrane fraction hardly changes	Cucumis sativus

Cloned(Commentary)

Cloned (Comment)	Organism
gene SUS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of plant SUS enzymes, real-time quantitative PCR enzyme expression analysis, antisense expression of SUS3 in Cucumber sativus plants transformed via Agrobacterium tumefaciens strain LBA4404	Cucumis sativus

Protein Variants

Protein Variants	Comment	Organism
additional information	antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance. Compared with the wild-type cucumbers, the transgenic lines with antisense expression of CsSUS3 are more sensitive to flooding. After 6 days of flooding, the enzyme activity, soluble sugar and UDP-glucose content and the ratio of ATP/ADP in the roots of transgenic plants are significantly lower than that in wild-type plants. The transgenic lines grow more slowly with more yellow necrosis in the leaves, phenotype overview	Cucumis sativus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Cucumis sativus	5829	-
plasma membrane	-	Cucumis sativus	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	the transcript level and protein abundance of CsSUS3 hardly change during salinity treatment, but the enzyme activity increased slightly, especially on the first and ninth days, compared with control	Cucumis sativus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
89000	-	x * 89000, about, sequence calculation	Cucumis sativus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NDP-glucose + D-fructose	Cucumis sativus	-	NDP + sucrose	-	r
NDP-glucose + D-fructose	Cucumis sativus Guonong No. 25	-	NDP + sucrose	-	r

Organism

Organism	UniProt	Comment	Textmining
Cucumis sativus	K9K7W3	-	-
Cucumis sativus Guonong No. 25	K9K7W3	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from roots partially by dialysis and ultracentrifugation	Cucumis sativus

Source Tissue

Source Tissue	Comment	Organism	Textmining
companion cell	-	Cucumis sativus	-
flower	female and male	Cucumis sativus	-
fruit	very low level	Cucumis sativus	-
phloem	-	Cucumis sativus	-
root	enzyme localization in the cells of phloem region in vascular tissues	Cucumis sativus	-
stem	-	Cucumis sativus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NDP-glucose + D-fructose	-	Cucumis sativus	NDP + sucrose	-	r
NDP-glucose + D-fructose	-	Cucumis sativus Guonong No. 25	NDP + sucrose	-	r

Subunits

Subunits	Comment	Organism
?	x * 89000, about, sequence calculation	Cucumis sativus

Synonyms

Synonyms	Comment	Organism
CsSUS3	-	Cucumis sativus
sucrose synthase 3	-	Cucumis sativus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Cucumis sativus

Expression

Organism	Comment	Expression
Cucumis sativus	the transcript level and protein abundance of CsSUS3 hardly change during salinity treatment, but the enzyme activity increased slightly, especially on the first and ninth days, compared with control	additional information
Cucumis sativus	when suffering hypoxia stress from flooding, CsSUS3 expression and SUS activity in roots increase, especially in the lateral roots. The soluble SUS activity increases, but the membrane fraction hardly changes. Drought stress modulated by PEG-6000 causes a transient and slight increase in CsSUS3 transcript level on the first and third days, but decreases similarly to control on the sixth and ninth days	up

General Information

General Information	Comment	Organism
malfunction	antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance	Cucumis sativus
physiological function	CsSUS3 participates in resisting hypoxic stress, mechanism analysis	Cucumis sativus

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Release 2023.1 (January 2023)