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Literature summary for 2.4.1.13 extracted from
- Sucrose-UDP glucosyltransferase of Zea mays endosperm (1974), Phytochemistry, 13, 885-891.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | sucrose cleavage, not synthesis | Zea mays |  |
| AMP | not inhibitory | Zea mays | |
| ATP | inhibition of cleavage, not synthesis | Zea mays | |
| Ca2+ | - |
Zea mays | |
| glucose | uncompetitive to UDPglucose | Zea mays | |
| Mg2+ | - |
Zea mays | |
| additional information | no inhibition by K+, Na+ | Zea mays | |
| UDP | at high concentrations; strong | Zea mays | |
| UTP | sucrose cleavage; synthesis | Zea mays | |
| Zn2+ | strong | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | 0.15 | UDP | - |
Zea mays | |
| 0.98 | 1.66 | fructose | cosubstrate UDPglucose, ADP | Zea mays | |
| 2.08 | 3.1 | fructose | - |
Zea mays | |
| 16.9 | 40 | sucrose | - |
Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition | Zea mays | |
| Ca2+ | activation of sucrose synthesis | Zea mays | |
| Mg2+ | activation | Zea mays | |
| Mg2+ | inhibition of sucrose cleavage | Zea mays | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 128 | - |
sucrose cleavage | Zea mays |
| 180 | - |
sucrose synthesis | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP + sucrose | - |
Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDPglucose + D-fructose | - |
Zea mays | UDP + sucrose | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 6.5: t1/2: 1 min, complete inactivation after 10 min, pH 8: 20% loss of activity after 1 min, 80% loss of activity within 10 min | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
sucrose cleavage | Zea mays |
| 8 | - |
sucrose synthesis | Zea mays |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
t1/2: 1 min, 55°C, complete inactivation within 10 min | Zea mays |
| 8 | - |
55°C: 20% loss of activity within 1 min, 80% loss of activity within 10 min | Zea mays |
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