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Literature summary for 2.4.1.13 extracted from
- A comparison of two sucrose synthetase isozymes from normal and shrunken-1 maize (1985), Plant Physiol., 79, 530-536.
General Stability
| General Stability | Organism |
|---|---|
| repeated freeze-thawing leads to slight decrease of activity, even in the presence of PMSF | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52 | 290 | sucrose | - |
Zea mays |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Zea mays |
| 87000 | - |
x * 87000, SDS-PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
maize, isozymes SS1, SS2, sh1/sh1- (Black Mexican Sweet) or sh1bz1-m4-genotype | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes: SS1 and SS2 | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Zea mays | - |
| endosperm | - |
Zea mays | - |
| seedling | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
isozyme SS2 | Zea mays |
| 10 | - |
isozyme SS1 | Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 20% glycerol, 0.1-1.0 mM DTT, stable | Zea mays |
| 4°C, prolonged storage leads to slight decrease of activity, even in the presence of PMSF | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP + sucrose | also substrate: ADP | Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | substrate: TDP | Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | with SS2-isozyme TDP is 57%, with SS1-isozyme 91% as effective as UDP | Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | ADP is less effective | Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDPglucose + D-fructose | - |
Zea mays | UDP + sucrose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 87000, SDS-PAGE | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.8, isozyme SS1 | Zea mays |
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